2015-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/687382摘要：藍莓 (Vaccinium spp.) 為目前世界上最受歡迎的新興小果類作物，經濟栽培之主要類別中，以南高叢 (V. hybrid) 及兔眼藍莓 (V. ashei) 各品種之低溫需求較低，且打破休眠臨界感應溫度高，可在臺灣平地行商業生產，惟多數品種於北美育成，耐熱生理及品種間耐熱差異相關研究甚少，對臺灣平地夏季高溫之耐受性仍待改進。傳統的耐熱性選拔及育種依賴對植株於田間生長發育的評估，對多年生的果樹之言，必需耗費極大的空間、時間、勞力和成本，若能輔以在高溫下植株特定部位生理指標變化，開發出準確、簡單及快速的耐熱性評估方法，將可大幅減省田間工作，有助於藍莓在台灣及氣候相似的亞熱帶地區栽培推廣及育種選拔。本研究擬1) 利用細胞膜熱穩定性及葉綠素螢光反應等實驗室技術，評估不同品種及品系之兔眼藍莓及南高叢藍莓耐熱性差異，2) 定量葉表解剖構造及葉片形態與耐熱性之相關性，及3) 調查開放授粉與人為雜交所產生之後代及親本各品種間在台灣平地高溫環境之下光合作用、水分生理及生長發育等相關數據，並與前述生理及形態指標比對，期望透過此一系列的研究，開發出可簡易且快速評估藍莓幼苗耐熱性之技術，用以提高苗期選拔效率，並藉此選育出更適合臺灣氣候及未來全球暖化環境的新品種。<br> Abstract: The blueberry (Vaccinium spp.) is currently the most popular and demanded small fruit crop. Among the major commercial blueberry categories, the low chilling required southern highbush (V. hybrid) and the rabbiteye blueberry (V. ashei) cultivars have shown potential to be cultivated in Taiwan’s lowland. However, the heat stress response for most cultivars has not been documented and their tolerance to the subtropical summer heat has yet to be improved. Conventional selections and breeding for heat tolerance require long-term and costly field trials. It is possible to accelerate the screening process by detecting the physiological or physiochemical responses of plant cell or tissue at the seedling stage in addition to growth and morphological monitoring. The objectives of this proposal are therefore 1) to evaluate differences in heat responses among currently available rabbiteye and southern highbush cultivars by membrane heat stability and chlorophyll florescence techniques, 2) to observe leaf micro-structure and morphology variations among cultivars and their responses to heat stress, and 3) to correlate mentioned traits with gas exchange and growth parameters and to apply these traits on open-pollinated and cross hybrid off springs. A simple and fast evaluation protocol for heat tolerance screening will be established to increase selection efficiency and to promote new cultivars with better performance in Taiwan’s subtropical climate and in future global warming conditions.葉綠素螢光細胞膜熱穩定氣體交換氣候變遷熱逆境生理chlorophyll florescencemembrane heat stabilitygas exchangeclimate changeheat stress physiology