2009-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/656943摘要：利用作物馴化的方式對照於其野生種可以來探討適應的遺傳基礎。水稻抗稻熱病 是因為真菌Magnopother grisea 的致病基因產物AVR-pita 與寄主之抗病Pi-ta 基 因蛋白質產物發生接觸而誘發的。野生稻(Oryza rufipogon) Pi-ta 基因之基因型 變異與抗病或是感病在本計劃內將加以調查以檢測寄主與病毒之共同演化與 Pi-ta 基因之功能性適應。為了研究水稻稻熱病之抗病與感病之分子族群遺傳的 基礎我們將利用親緣地理的研究方式。此外，我們也將利用微陣的技術針對野生 稻與栽培稻抗稻熱病Pi-ta 基因所誘導與抑制的基因种類加以調查，並用來評估 分子層次的適應性演化.<br> Abstract: Crop domestication provides a lot of chance to study the genetic basis of adaptation evolution of many characters in relation with its wild relatives. Rice blast disease resistance to the fungal pathogen Magnopothe grisea is triggered by the physical interaction between the protein products of the host resistance (R) gene Pi-ta and the pathogen avriulence gene AVR-pita. The genotype variation and resistant/susceptible phenotype at the Pi-ta locus of wild rice O. rufipogon, the ancestor relative of rice O. sativa, will be surveyed in this study to test hypotheses of host-parasite coevolution and function adaptation of the Pi-ta gene. A phylogeographical approach will be used to study the molecular population genetic basis of rice blast resistance and sensitivity. In addition, microarray approach will be used to investigate the genes upregulated and downregulated in blast resistance Pi-ta gene in wild rice and cultivated rice, so that the adaptive evolution can be evaluated at the molecular level.Pi-ta 基因野生稻親緣地理適應性演化Pi-ta geneOryza rufipogonphylogeographyadaptive evolution