2023-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/654300綠豆是亞洲的重要作物，為發展中國家的小農提供重要經濟來源以及便宜的植物性蛋白質和營養來源。儘管如此，促進綠豆育種所需的基因體工具和資源目前仍相對缺乏。在本研究，我們與國際綠豆改良研究網絡和數個主要作物種原中心合作，預計深入探究此重要作物的基因體多樣性。以本團隊堅實的先期結果和數個高品質參考基因體序列為基礎，我們首先會將超過1400個品系以及超過1800個人工雜交後代的基因體定序資料定位到本團隊組裝的高品質參考基因體上。使用同一個參考基因體，未來不同國際團隊的基因定位結果可以直接互相比較，加速後續育種進程。我們的先期研究發現綠豆在印度被馴化後，循著一個獨特的路徑依序擴散到東南亞、東亞、中亞。亞洲各地的環境差異很有可能塑造了這個獨特的傳播路徑，而且我們更發現中亞綠豆演化出更高的地下部/地上部重量比以適應當地的乾燥環境。利用我們手上的全球遺傳多樣性資訊，我們預計研究綠豆馴化過程中的族群動態、進行地下部/地上部重量比以及其他重要農藝性狀的遺傳定位、最後利用基因體選拔法預測全球種原的性狀數值以及適合的栽種環境。本研究不僅能讓我們理解綠豆馴化的過程以及其中的人擇和天擇壓力，更能加速國際團隊對此重要作物的改良進程。 Mungbean (Vigna radiata) is an important legume in Asia, providing incomes and cheap plant-based proteins and nutrients especially for smallholder farmers in developing countries. Despite so, the genomic tools and resources necessary to facilitate breeding are currently scarce. In collaboration with the International Mungbean Improvement Network and major crop stock centers of the world, here we propose to deepen the investigation of genomic variation in this essential crop. Based on our solid preliminary results and several high-quality reference genomes, we will map the genome-sequencing data from >1400 accessions and >1800 nested association mapping (NAM) progenies on the same high-quality reference genome we assembled. This will allow the direct comparison of genetic mapping results among international teams and will be a solid foundation for all downstream breeding efforts. Our previous results have shown that after being domesticated in India, mungbean followed a unique expansion route, reaching Southeast Asia, East Asia, and Central Asia subsequently. This unique expansion route was likely constrained by the environmental differences across Asia, and the evolution of higher root/shoot ratio may allow the successful cultivation of mungbean in the arid Central Asia. Using this comprehensive collection of global genetic variation, we will investigate the demographic history during mungbean domestication, perform genetic mapping for root/shoot ratio and other agronomic traits, and conduct genomic selection to predict the phenotypes and suitable cultivation environments for worldwide accessions. The results will not only help us understand the process and selection pressures during crop domestication but will also greatly facilitate international breeding progress of this essential crop.綠豆;馴化;全基因體關聯性定位;基因體選種;族群基因體學;mungbean; domestication; genomewide association study; genomic selection; population genomics