SUI-YUAN CHANGLee C.-N.Lin P.-H.Huang H.-H.LUAN-YIN CHANGKo W.Chang S.-F.PING-ING LEELI-MIN HUANGCHUAN-LIANG KAO2020-07-032020-07-0320080146-6615https://scholars.lib.ntu.edu.tw/handle/123456789/508025An outbreak of respiratory adenovirus infection in children was observed in northern Taiwan between November 2004 and February 2005. Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to determine the serotype(s) of 172 adenovirus isolates in the outbreak period, we found that adenovirus type 3 (Ad3) was the predominant type (87.2%), followed by Ad2 (6.4%), Ad 1 (4.1%), Ad7 (1.2%), Ad4 (0.6%), and Ad5 (0.6%). The genotype of Ad3 was analyzed for 15 isolates from the outbreak period by RFLP of the full-length genome. All these isolates belonged to genotype Ad3a2. Compared with the Ad3-infected patients in the baseline period, a significantly higher proportion of Ad3-infected patients in the outbreak period had severe infections (58.0% vs. 40.2%, P = 0.01), which included bronchopneumonia (28.7%), exudative tonsillitis (24.1%), and tonsillitis (16.1%). Moreover, patients with severe infections were significantly younger than those without (4.10 vs. 8.15 years, P < 0.001). In summary, our study demonstrated that Ad3 was the predominant serotype responsible for the respiratory adenovirus outbreak in northern Taiwan during 2004-2005 and was associated with severe infections in the outbreak period. ? 2007 Wiley-Liss, Inc.[SDGs]SDG3adenovirus type 3 infection; animal cell; article; bronchopneumonia; genotype; human; human cell; nonhuman; nucleotide sequence; polymerase chain reaction; restriction fragment length polymorphism; serotype; Taiwan; tonsillitis; virus infection; virus isolation; Adenovirus Infections, Human; Adenoviruses, Human; Antigens, Viral; Child; Disease Outbreaks; DNA, Viral; Epidemiology, Molecular; Humans; Pharynx; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Serotyping; Taiwan; Adenoviridae; Human adenovirus type 3journal article10.1002/jmv.21045180410262-s2.0-36849077361