Chang C.-C.Lin S.Lee C.-H.Chuang T.-L.PO-REN HSUEHHSIN-CHIH LAILin C.-W.2020-12-182020-12-1820120956-5663https://scholars.lib.ntu.edu.tw/handle/123456789/528444Interferon-gamma (IFN-γ) is associated with susceptibility to tuberculosis, which is a major public health problem worldwide. Although significant progress has been made with regard to the design of enzyme immunoassays for IFN-γ, this assay is still labor-intensive and time-consuming. We therefore designed a DNA aptamer hairpin structure for the detection of IFN-γ with high sensitivity and selectivity. A streptavidin DNA aptamer was incorporated into the IFN-γ binding aptamer probe for the amplified detection of the target molecules. Initially, the probe remained in the inactive configuration. The addition of IFN-γ induced the rearrangement of the aptamer structure, allowing the self-assembly of the active streptavidin aptamer conformation for the streptavidin molecular recognition. Under optimized conditions, the detection limit was determined to be 33. pM, with a dynamic range from 0.3 to 333. nM, both of which were superior to those of corresponding optical sensors. Because combined aptamers are composed of nucleic acids, this optical aptasensor provided the advantages of high sensitivity, simplicity, reusability, and no further labeling or sample pre-treatment. ? 2012 Elsevier B.V.[SDGs]SDG3Aptamers; Detection limits; Dynamic range; Enzyme immunoassay; Hairpin structures; High sensitivity; Interferon-gamma; Optimized conditions; Sample pretreatment; Streptavidin; Surface plasmon resonance immunosensors; Target molecule; Antibiotics; Glycoproteins; Immunosensors; Molecular recognition; Nucleic acids; Probes; Reusability; Surface plasmon resonance; Proteins; aptamer; DNA aptamer; gamma interferon; streptavidin; unclassified drug; analytical equipment; article; controlled study; DNA probe; DNA structure; immunosensor; intermethod comparison; limit of detection; molecular recognition; optical biosensor; protein analysis; protein conformation; reaction optimization; sensitivity and specificity; surface plasmon resonance; Aptamers, Nucleotide; Base Sequence; Binding Sites; Humans; Interferon-gamma; Limit of Detection; Nucleic Acid Conformation; Streptavidin; Streptomyces; Surface Plasmon Resonancejournal article10.1016/j.bios.2012.04.038226268292-s2.0-84861760572