Pai, Yun LingYun LingPaiWu, Yueh FengYueh FengWuSUNG-JAN LIN2023-08-252023-08-252022-11-109781450397223https://scholars.lib.ntu.edu.tw/handle/123456789/634753The transparent corneal stroma, which consists of regularly packed collagen fibrils and keratocytes, comprises 90% of the corneal thickness. Injury response in corneal stroma compromises corneal transparency due to overproduction of abnormal extracellular matrix by keratocyte-myofibroblast transformation. Corneal transplantation is considered the optimal solution to restore vision. Whether the keratocyte-myofibroblast transformation can be reversed remains unclear. In this study, we first developed a mouse corneal mechanical injury model. At 24h after the injury, α-SMA expression significantly increased, and SHG signals changed with respect to the collagen orientation. In addition, we isolated primary human keratocytes in advance; thereafter, we can decipher the keratocytes-myofibroblast transformation by adding TGF-β1 in the future. This proposal established three platforms, including a mouse injury model, primary human cell culture, and transgenic mice for targeting keratocytes. We will integrate these systems with single-cell transcriptomics, ATAC-sequencing, and proteomics to comprehensively explore keratocyte-myofibroblast transformation, and eventually, identify therapeutic targets for treating corneal fibrosis.Corneal opacification | fibrosis | keratocyte | myofibroblast | scar | wound healingconference paper10.1145/3574198.35742352-s2.0-85150360253https://api.elsevier.com/content/abstract/scopus_id/85150360253