2014-02-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/685060摘要：本計畫目的為確認錦鯉疱疹病毒抗原性，發展快速診斷套組，找出檢測病毒最佳時間。以罹病後錦鯉血清與病毒進行蛋白質二維電泳分析，找出能誘發魚體產生免疫反應的抗原，以提供生產抗體時優先抗原之選擇。另外採取田間發病之錦鯉病例，實際測試找出最佳檢測時間及最適宜檢測樣品。預期可縮短錦鯉疱疹病毒診斷時間，避免病毒擴散，提高我國錦鯉外銷品質，增加農民之收入。<br> Abstract: The aim of this project is to realize the antigenicity of koi herpesvirus, to develop a diagnostic kit and to determine the optimal time to detect the virus. First, the antigen reacted with antiserum from fish surviving the outbreak will be defined using the 2-D electrophoresis and Western blot to provide candidates of the antigen used in antibody production. In addition, the samples from the KHV-infected carp during outbreak will be used to find out the optimal detection time and suitable samples for test. The expected results can shorten the diagnostic time for KHV, avoid viral spreading in field, upgrade the quality of koi for export, and increase the farmers＆apos; income.診斷試劑抗原性分析錦鯉疱疹病毒diagnostic kitantigenicity analysiskoi herpesvirus錦鯉疱疹病毒快速診斷驗劑開發