2002-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/688934摘要：德國蜚蠊雌成蟲在活動行為的表現上，會因卵巢的發育而出現活動行為日週律動被掩蓋的現象。由於德國蜚蠊卵巢的掩蓋效應是由荷爾蒙系統所調控，推測卵巢所分泌最大量的激素「蛻皮激素」就是掩蓋因子。首先藉由檢測不同日齡德國蜚蠊成蟲體內蛻皮激素濃度，可以知道蛻皮激素濃度變化與掩蓋效應的產生是否有對應的關係: 接著以免疫染色的方法找出蛻皮激素在德國蜚蠊成蟲體內作用之位置。然後將雌成蟲的卵巢除去再以人工合成的蛻皮激素類似物處理，證實蛻皮激素確實可以產生掩蓋效應。前述實驗流程在雙紋姬蠊也進行相同的操作。由於德國蜚蠊與雙紋姬蠊處女雌蟲的日週律動表現上出現差異，比較這兩種蜚蠊之蛻皮激素與掩蓋效應的關係，可以了解造成日週律動表現在種間差異的生理機制為何。最後以分子生物技術產生不具有蛻皮激素的成蟲個體，從基因層面探討蛻皮激素與掩蓋效應的關係。<br> Abstract: The expression of locomotor circadian rhythm in female German cockroach is masked by the development of ovaries. Since the masking effect is mediated through the regulation of hormone, we propose the ecdysone, the major hormone secreted from ovaries, is the masking factor. Firstly, by monitoring the ecdysone titer in different aged females, we can establish the base line of ecdysone along with its reproductive cycles. This result can be correlated with the masking effect on the locomotor circadian rhythm. In addition, we will employ immunostain method against ecdysone receptor to reveal the action sites. These sites may shine light on the interference of circadian signals with masking factor-ecdysone. In order to prove the masking role of ecdysone, ovariectomized females who showed unmasked locomotor circadian rhythm will be treated with ecdysone analog to regain the masked rhythm. Since the sibling species of the Germany cockroach, the double-striped cockroach is a feral species and expresses locomotor circadian rhythm in virgin females, it is a great advantage to use this species as a comparative study to further demonstrate the function of ecdysone on the locomotor circadian rhythm. The same experimental procedures, therefore, will be repeated on the double-striped cockroach. The main focus of this proposal is to investigate the physiological mechanism of masking effect on the locomotor circadian rhythm by ecdysone. Furthermore, the molecular mechanism of masking effect by ecdysone will be also demonstrated by abolishing ecdysone production at the mRNA level with RNA interference technique.德國蜚蠊雙紋姬蠊活動行為日週律動掩蓋效應蛻皮激素Germany cockroachdouble-striped cockroachlocomotor circadian rhythmmasking effectecdysone