2019-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/651807摘要：自然界中鬥爭行為對動物的生存非常重要，動物經由鬥爭行為爭奪食物、交配對象、領域等資源，個體之間藉由打鬥行為建立階級，以維持族群的安定，然而我們對於調控鬥爭行為的神經迴路所知有限。我們過去的研究結果指出，兩個區域的背側韁核神經元互為結抗地調控動物的鬥爭行為：背側韁核的外側區域負責維持打鬥行為，背側韁核的內側區域則與終止打鬥行為相關。由於背側韁核的外側區域將神經訊號送至背側椎間核，背側椎間核再將訊號送至中央灰層&#8237;;&#8236;背側韁核的內側區域將神經訊號送至腹側椎間核，腹側椎間核再將訊號送至中央背縫，因此我們認為韁核迴路中的各核區，包含韁核、椎間核、中央灰層與中央背縫皆參與動物鬥爭行為的調控。本計畫將以動物行為學與神經生物學的策略，在未來三年進行以下研究重點：一、藉由追蹤神經纖維與標定神經傳導物質，研究背側椎間核與腹側椎間核之間互相調控的關係。二、操控椎間核活性，研究其在鬥爭行為上扮演的角色。三、解析外界環境經由影響韁核迴路，調控鬥爭行為之機制。<br> Abstract: Aggression is an evolutionary conserved behavior that is critical for animals’ survival and exists in different animal taxa. To compete limited resources such as food, mating chance, and territory, animals establish a social hierarchy through aggressive and fighting behaviors to maintain the stability of a population. However, the mechanisms by which neural circuits regulate aggressive behaviors remain largely unknown. We previously showed that the lateral and medial subnuclei of the dorsal habenula (dHbL and dHbM) antagonistically regulate the outcome of social conflicts. The dHbM projects the ventral interpeduncular nucleus (vIPN) and intermediate IPN (iIPN), whereas the dHbL sends axons to the dorsal IPN (dIPN) . The dIPN further projects the axons toward the griseum centrale (GC) while the vIPN projects to the median raphe (MR). Based on these neural connections, we hypothesize that the habenular circuit including the habenula, IPN, GC, and MR are all involved in the regulation of fighting behaviors. In the coming three years, we will use behavioral and neuroscience approaches to achieve the specific aims: (1) Trace the neural projections from the dIPN and vIPN and characterize the cell types of these neurons to elucidate the integration mechanisms of conflict decision between the dIPN and vIPN. (2) Manipulate the neural activities in the vIPN genetically and/or pharmaceutically and investigate the neural activities in the MR and GC and their effects on fighting behaviors. (3) Investigate the mechanisms of environmental effects on fighting behaviors through regulating the habenular circuit.疆核鬥爭行為HabenulaAggressive behavior