2013-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/705426摘要：在高鹽逆境下，作物產量大大地減少。植物通過調整各項相關信號轉導路徑對鹽逆 境產生反應，此包括離層酸(ABA)依存及離層酸獨立的途徑。WRKY 是植物的特有的轉錄 因子，可以結合到目標基因啟動子的W-框(W-box)行轉錄調控。在以前的研究中，AtWRKY 發現參與鹽或滲透逆境的反應和抗性。此外，AtWRKY 發現也參與ABA 信號傳導。在我 們的初步結果，我們發現一些 AtWRKY 基因的過度表達之阿拉伯芥植株是耐鹽的。此 外，一些 AtWRKY 基因的過度表達阿拉伯芥植株對離層酸有不同的敏感度。我們推測， WRKY 基因對鹽或滲透逆境的反應發揮重要作用，並參與植物體內的離層酸的信號傳導。 在此計畫中我們使用阿拉伯芥作為模式生物。此計畫的最終目標是要闡明WRKY 參與鹽 或滲透逆境的反應及抗性機制，和在離層酸信號傳導中的作用。35S 啟動子驅動的 AtWRKY 過量表達植株將建立，並測試受到鹽、滲透壓力或離層酸處理之回應。特別是， 我們想觀察根的外表型。為了確定受鹽或滲透壓力影響之下游基因或蛋白質，將進行微 陣列及蛋白質組學分析。我們將使用晶片分析使用AtWRKY 突變株在鹽、滲透壓力或離 層酸處理下揭示下游受調控之基因。我們將推出蛋白質組學分析比較野生型及突變線下 鹽、滲透壓力或 離層酸處理下之蛋白質積累。我們希望這項研究將導致更好地瞭解WRKY 基因的作用機轉，我們希望我們最終可以應用於農作物(如水稻)改善鹽逆境抗性和提高 作物產量。 <br> Abstract: Under salinity stress, crop yield is significantly reduced. Plants respond to salt stress by adjusting various signaling transduction pathways including abscisic acid (ABA)-dependent and ABA-independent pathways. WRKY is a plant specific transcription factor which can bind to W-box of target gene promoter for transcriptional regulation. In previous studies, AtWRKY was found to be involved in salt or osmotic stress response and tolerance. In addition, AtWRKY was found to be involved in ABA signaling pathway. In our preliminary results, we have found some AtWRKY gene overexpression lines are salt-tolerant. In addition, some AtWRKY gene overexpression lines showed differential sensitivity to ABA. We hypothesize that WRKY play important role in salt or osmotic stress response, and involve in ABA signaling pathway in planta. In this proposed project we use Arabidopsis thaliana as model system. The ultimate goal of this project is to elucidate the function of AtWRKY in salt or osmotic stress response, and ABA signal transduction pathway. Over-expression line of WRKY driven by 35S promoter will be generated and subjected to phenotyping to observe salt, osmotic stress, or ABA response. In particular, we would like to observe root phenotype. In order to identify downstream genes or proteins affected by salt or osmotic stress, transcriptomic and proteomic analysis will be introduced. We will use microarray analysis to reveal the up-regulated and down-regulated genes using knockout mutant wrky and over-expression line under salt, osmotic stress, or ABA treatment. A 2D-gel based and gel-free proteomic analysis will be introduced to compare protein accumulation in wild type and mutant line under salt, osmotic stress, or ABA treatment. We hope that this study will lead to a better understanding of the function of WRKY gene in salt stress response and tolerance. We hope we can eventually be applied to crop (i.e. rice) to improve salt stress resistance and improve crop yield.離層酸鹽WRKYABAsaltWRKY