Liu, Yi-HsinYi-HsinLiuTsai, Yi-YunYi-YunTsaiChien, Hsiao-JuHsiao-JuChienChen, Chien-YingChien-YingChenHuang, Yu-FengYu-FengHuangChen, Jinn-ShiunJinn-ShiunChenWu, Yi-ChunYi-ChunWuChen, Chia-ChunChia-ChunChen2012-10-192018-07-062012-10-192018-07-06201109574484http://ntur.lib.ntu.edu.tw//handle/246246/243294http://ntur.lib.ntu.edu.tw/bitstream/246246/243294/-1/33.pdfhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-79952682713&doi=10.1088%2f0957-4484%2f22%2f15%2f155102&partnerID=40&md5=318740f2e2e2cfb564bd920d7b8b788fWe have developed a new technique using fluorescent silica nanotubes for simple and sensitive DNA detection. The quantum-dot-embedded silica nanotubes (QD-SNTs) were fabricated by a sol-gel reaction using anodic aluminum silica oxide (AAO) as a template. The fluorescent QD-SNTs of different colors were then immobilized with single-stranded DNA and used as nanoprobes for DNA detection. The optical and structural properties of QD-SNT nanoprobes were examined using photoluminescence spectroscopy, confocal microscopy and transmission electron microscopy (TEM). The QD-SNT nanoprobes were applied to detect dye-labeled target DNA in a solution phase. The obvious color change of the QD-SNT nanoprobes was observed visually under a simple microscope after the successful detection with target DNA. The quantitative analyses indicated that ∼ 100 attomole of target DNA in one nanoprobe can generate a distinguishable and observable color change. The detection results also demonstrated that our assay exhibited high specificity, high selectivity and very low nonspecific adsorption. Our simple DNA assay based on QD-SNT nanoprobes is expected to be quite useful for the needs of fast DNA screening and detection applications. © 2011 IOP Publishing Ltd.en-USAttomole; Color changes; DNA assays; DNA detection; DNA screening; High selectivity; High specificity; Nonspecific adsorption; Quantitative analysis; Quantum dots; Silica nanotubes; Silica oxide; Single-stranded DNA; Sol-Gel Reaction; Solution phase; TEM; Adsorption; Anodic oxidation; Color; Colorimetry; Confocal microscopy; DNA; Fluorescence; Nanoprobes; Nanotubes; Photoluminescence spectroscopy; Semiconductor quantum dots; Silica; Transmission electron microscopy; Nucleic acids; 5 carboxytetramethylrhodamine succinimidyl ester; 5-carboxytetramethylrhodamine succinimidyl ester; carbocyanine; complementary DNA; cyanine dye 3; DNA; fluorescein isothiocyanate; fluorescent dye; nanotube; quantum dot; rhodamine; silicon dioxide; adsorption; article; chemistry; DNA probe; fluorescence microscopy; nucleic acid hybridization; particle size; spectrofluorometry; transmission electron microscopy; Adsorption; Carbocyanines; DNA; DNA Probes; DNA, Complementary; Fluorescein-5-isothiocyanate; Fluorescent Dyes; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Nanotubes; Nucleic Acid Hybridization; Particle Size; Quantum Dots; Rhodamines; Silicon Dioxide; Spectrometry, Fluorescencejournal article10.1088/0957-4484/22/15/15510210.1088/0957-4484/22/15/15510210.213895772-s2.0-79952682713WOS:000288209700002http://ntur.lib.ntu.edu.tw/bitstream/246246/243294/-1/33.pdf