MIN-SHU HSIEHLee Y.-H.Jin Y.-T.Huang W.-C.2020-03-052020-03-0520180046-8177https://www.scopus.com/inward/record.uri?eid=2-s2.0-85055755557&doi=10.1016%2fj.humpath.2018.07.026&partnerID=40&md5=c1b4c8f7b6643725ed77b27add0c7d22https://scholars.lib.ntu.edu.tw/handle/123456789/470761Human papillomavirus (HPV)–related multiphenotypic sinonasal carcinoma (HMSC) is associated with high-risk HPV (HR-HPV) infection. Using HR-HPV messenger RNA (mRNA) in situ hybridization (ISH), we reported 6 new HMSC cases and compared their histopathology with that of sinonasal adenoid cystic carcinoma. Using p16 immunohistochemistry (IHC) and HR-HPV ISH, we retrospectively identified 6 HMSC cases. All HMSC cases were positive for HR-HPV mRNA ISH and p16 IHC. Two HMSC cases had overlying atypical squamous epithelium, and 1 had invasive squamous cell carcinoma (SCC). All HMSC cases were SOX10 positive, whereas the overlying atypical squamous epithelium and the SCC were SOX10 negative. One atypical HMSC-like case was also identified, which was positive for HR-HPV mRNA ISH, HR-HPV DNA ISH, and SOX10 IHC, but negative for p16 IHC. This study showed that HR-HPV mRNA ISH was a useful tool to diagnose HMSC and had stronger signals compared with HR-HPV DNA ISH. HR-HPV E6/E7 mRNA could be identified in the overlying atypical squamous epithelium and the invasive SCC. A combination of p16 and SOX10 IHC will be a useful screening panel for HMSC followed by confirmatory HR-HPV mRNA ISH test. ? 2018 Elsevier Inc.Adenoid cystic carcinoma; HPV-related multiphenotypic sinonasal carcinoma; mRNA ISH; P16; SOX10[SDGs]SDG3beta catenin; messenger RNA; protein Myb; protein p16; transcription factor Sox10; virus DNA; virus RNA; cyclin dependent kinase inhibitor 2A; messenger RNA; P16 protein, human; SOX10 protein, human; transcription factor Sox; tumor marker; virus RNA; adenoid cystic carcinoma; adult; aged; Article; basement membrane; cancer diagnosis; clinical article; comparative study; female; fluorescence in situ hybridization; gene rearrangement; histopathology; human; human cell; human tissue; immunohistochemistry; invasive carcinoma; keratinization; male; middle aged; multiphenotypic sinonasal carcinoma; negative staining; papillomavirus infection; protein expression; retrospective study; salivary gland cancer; squamous cell carcinoma; squamous epithelium; tumor necrosis; adenoid cystic carcinoma; biopsy; chemistry; fluorescence in situ hybridization; genetics; immunohistochemistry; in situ hybridization; Papillomaviridae; papillomavirus infection; paranasal sinus tumor; pathology; predictive value; reproducibility; virology; Adult; Aged; Biomarkers, Tumor; Biopsy; Carcinoma, Adenoid Cystic; Cyclin-Dependent Kinase Inhibitor p16; Female; Humans; Immunohistochemistry; In Situ Hybridization; In Situ Hybridization, Fluorescence; Male; Middle Aged; Papillomaviridae; Papillomavirus Infections; Paranasal Sinus Neoplasms; Predictive Value of Tests; Reproducibility of Results; Retrospective Studies; RNA, Messenger; RNA, Viral; SOXE Transcription Factorsjournal article10.1016/j.humpath.2018.07.026300712332-s2.0-85055755557