2004-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/662214摘要：樟芝(Antrodia camphorate)，是臺灣特有之珍貴藥用菌種，生長於保育類牛樟的中空心材內壁上，由於樟芝成分相當複雜，菌種難以馴服，包括寄主、營養成份及環境的溫溼度等皆會影響其生長發育。樟芝活性成分，包括三&#33820;類、多醣體等，可能具有多方面如抗癌或調節免疫等活性，然而與有關基因層面的研究則尚未有報告發表。因此，本計畫則將是著眼於樟芝出菇之調控基因，利用扣除雜交及現代蛋白質體學分析技術選殖菌絲體與子實體表現差異之基因群，尋找與出菇表現有關之蛋白質，並鑑定出與三&#33820;類生合成有關的基因後，達成調控樟芝出菇的 目標，進而減少保育類牛樟的盜伐，以及藥物開發之應用。<br> Abstract: Chang-Zhi (Antrodia camphorate) is an endemic fungal species grown in Taiwan. It only grow on the inner of the Cinnamomum kanehirai decay heartwood. A. camphorata has been reported to have many medicinal uses, including anticancer and immunomodulating activities due to the active constituents of this fungus, especially, the group of triterenoids and polysaccharides. However, basic molecular biology study of A. camphorate is still weak. The objective of this proposal is focused on identifying genes involved in the regulation of fruit body development. We believe that the results obtained in this study would provide the approaches lead to the improvement of the fruiting of A. camphorate, protection the C. kanehirai, and drug discovery in the future.樟芝出菇雙向電泳法表現差異Antrodia camphoratefruiting2-D electrophoresisdifferentially expression