陳義雄2006-07-252018-07-062006-07-252018-07-062005-07-31http://ntur.lib.ntu.edu.tw//handle/246246/10255執行本專題時，從儲精囊液中發現了一個34 kDa 的醣蛋白，進一步鑑定該蛋白是 Ceacam 10 基因的衍生物。此蛋白主要表現於儲精囊，其他生殖腺的表現則十分少。該蛋 白於儲精囊內腔表皮細胞的表現與發育有關，受到雄性素的調控。進一步也證實該蛋白可 以促進精子的游動。CEACAM10 was purified from mouse seminal vesicle secretion by a series of purification steps including ion exchange chromatography on a DEAE-Sephacel column and ion exchange HPLC on an SP column. It was shown to be a 36-kDa glycoprotein with an N-linked carbohydrate moiety. The CD spectrum of CEACAM10 in 50 mM phosphate buffer at pH 7.4 appeared as one negative band arising from the β-form at 217 nm. In the reproductive glands of adult mice, CEACAM10 was expressed predominantly in seminal vesicles. Both CEACAM10 and its mRNA were demonstrated on the luminal epithelium of the mucosal folds in the seminal vesicle. The amount of Ceacam10 mRNA in the seminal vesicle was correlated with the stage of animal maturation. Castration of adult mice resulted in cessation of Ceacam10 expression, while treatment of the castrated mice with testosterone propionate in corn oil restored the Ceacam10 expression in the seminal vesicle. During the entire course of pregnancy, Ceacam10 might be silent in the embryo. A cytochemical study illustrated the presence of the CEACAM10-binding region on the entire surface of mouse sperm. CEACAM10-sperm binding greatly enhanced sperm motility in vitro.application/pdf167229 bytesapplication/pdfzh-TW國立臺灣大學生化科學研究所reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/10255/1/932311B002011.pdf