2018-01-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/661607摘要：神經系統疾病由於再生性受限，因此相關之外傷或病變所造成的神經損傷，往往造成永久性而無法恢復之神經障礙，影響個體社會功能甚鉅。如果能在神經受損的早期予以有效的治療，神經的保護與再生是可能的。幹細胞治療近年已帶給相關神經一線曙光，但是如何提升細胞的分化與安定性，以及高效率之手術移植，成為當務之急。視神經是中樞神經的起點，我們將由視神經再生的研究開始再延伸至腦神經再生。本計畫在此提出組織工程研究，希望利用生物可相容支架的方法，促進神經性幹細胞的生長與分化，並兼具層狀載體與軸突引導功能，促進視神經損傷後，神經再生之解剖與功能性恢復。這是以團隊進行之長程研究，我們目前已建立相關幹細胞分化基礎，利用合成蛋白質支架協助神經幹細胞軸突延伸之成果，以及高專一性之動物實驗模型。本團隊在此提出三年期計畫，目標在於完成：(1) 建立人體視網膜神經節幹細胞分化培養方法，(2) 發展生物可相容，且具神經促進能力的3D&#32957;高分子/導電高分子支架，及 (3) 以高度獨特功能性之轉殖基因鼠品種進行動物解剖與功能性實驗，驗證幹細胞支架複合物提供神經的修復與再生功能。本計畫團隊以台灣大學分子生醫影像中心團隊為主體，結合跨領域之專家，包括工學院材料系教授林唯芳、羅世強，化工系教授游佳欣；另加入京都大學木村教授，高分子物理專家;中研院神經幹細胞培養之專家唐堂特聘研究員技術協助；更結合美國哈佛大學醫學院眼科陳東風教授第一線之視神經再生研究技術與視野，期望在此三年計畫下，達成幹細胞-聚合物支架複合物研發成功以及臨床前期實驗之完成。我們相信，此研究團隊具有高度國際競爭力，並且可產出具有世界領導地位之研究成果。<br> Abstract: The regenerative ability is limited in our nervous system. Therefore, neuropathy usually leads to permanent disturbance; detract biological and social functions. If prompt treatments are available in the early stage after nerve damage, the neural protection and even regeneration is possible. The stem cell therapy has raised a silver lining for these patients. Nowadays, the major issue we encounter is to promote the differentiation and stability of neural stem cells and to develop a high-efficiency technique of nerve transplantation. The optic nerve is the starting point of central nerve system. We will begin with the optic nerve regeneration, then extend to the brain. This proposal focuses on the tissue engineering approach for never regeneration. We are developing bio-compatible scaffold to facilitate stem cell differentiation, axon extension, and laminar arrangement. We hope the stem cell-scaffold complex would promote the anatomical resuscitation and functional recovery after optic nerve damage. This is a teamwork and we have achieved (a) in vivo differentiation of retinal ganglion cell (RGC)-like stem cells, (b) peptide-assisted axon extension of these cells, and (c) animal model with high specific transgenic mice. The presenting proposal is a three-year project, and our goals are: (1) to establish the protocol of efficient RGC differentiation from stem cells, (2) to develop highly bio-compatible and neural-promoting polypeptide, polyelectrolyte/conducting polymer scaffolds, and (3) to evaluate the neural protective effect of the stem cell-scaffold complex via transgenic mice experiments. The multi-discipline team is based on the members of NTU-MIC (National Taiwan University, Molecular Imaging Center), including the Distinguished Professor Wei-Fang Su / Shyh-Chyang Luo of Department of Materials Science and Engineering, Prof. Chia-Shin Yu of Department of Chemical Engineering, Dr. Ta-Ching Chen of Ophthalmology Department, College of Medicine. Prof. TK Tang, a Distinguished Researcher of Academic Sinica, will also join the work of neural stem cells, Prof. T. Kimura of Kyoto University, a distinguished polymer physicist. We have set long-term cooperation with Prof. Dong-Feng Chen of Harvard Medical School to make sure the frontier perspectives in the field of optic nerve regeneration. After the completion of the three-year project, we anticipate to achieve the development of effective stem cell-scaffold complex and complete the pre-clinical trials both in vitro and in vivo. We believe that our team has the ability to achieve the world-leading results in this research field.神經再生眼睛腦幹細胞組織工程Keywords: nerveregenerationtissue engineeringopticstem cell.