Pai, Su-ChengSu-ChengPaiYang, Chung-ChengChung-ChengYangRiley, J. P.J. P.Riley2009-12-092018-06-282009-12-092018-06-281990http://ntur.lib.ntu.edu.tw//handle/246246/173762https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025355530&doi=10.1016%2fS0003-2670%2800%2985116-8&partnerID=40&md5=3fd5055e785f49d1337bc0aa652b9281The effects of variation of acidity and molybdate concentration on the spectrophotometric determination of phosphate by the method of Murphy and Riley were studied. The [H+]:[Mo] ratio was found to be a crucial parameter which not only influences the form of the final reduced complex, but also plays a key role in controlling the reaction kinetics. Normal colour formation of the reduced phosphoantimonylmolybdenum blue complex was only observed with a ratio between 60 and 80; the reaction was faster at low acidities. Silicate is a possible source of interference at high temperatures, but the formation of the silicoantimonylmolybdenum blue complex was found to be much slower than that of the corresponding phosphorus species. © 1990.application/pdf504235 bytesapplication/pdfen-USmolybdenum; phosphate; article; assay; methodology; nonhuman; ph; spectrophotometryjournal article10.1016/S0003-2670(00)85116-82-s2.0-0025355530WOS:A1990CQ48300015http://ntur.lib.ntu.edu.tw/bitstream/246246/173762/1/07.pdf