Kuo C.-T.Chiang C.-L.Wu C.-T.Huang G.-S.RUBY YUN-JU HUANGLee H.Wo A.M.2020-06-262020-06-262012#VALUE!https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901769517&partnerID=40&md5=fa8f33f66ed3ab7bb9ae450c25b0a0f8https://scholars.lib.ntu.edu.tw/handle/123456789/504755Screen for drugs that specifically kill epithelial cancer stem cells (CSCs) has not yet been realized due to the rarity of these cells within tumor cell population and their instability in culture [1]. Here we present a microfluidic chip that can facilitate culture and enrichment of cells with CSC-like properties in vitro. Furthermore, we show that human epithelial cancer cells can grow to a tumor spheroid, acquire properties of mesenchymal cells (an increased ability of migration), and have a gain of CSC properties (an increased drug resistance). In addition, this chip requires no external pumps and tubings yet being pipette-friendly in usage.Cancer stem cell; Drug resistance; EMT; Microfluidic; Pipette-friendly; Spheroid[SDGs]SDG3Cell culture; Cell proliferation; Microfluidics; Stem cells; Cancer stem cells; Drug resistance; EMT; Pipette-friendly; Spheroid; Diseasesconference paper2-s2.0-84901769517