2012-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/658787摘要：背景與目的：傳統上認為使用低頻超音波於超音波導入是增進藥物經皮傳輸最有效的 方式，目前認為增加皮膚通透性的主要機轉可能為超音波導入時產生的空泡效應。而 體外震波比多數其他形式的醫用超音波較能有效產生短暫的空泡，但僅有少量的熱效 應，因此震波可能較超音波更合適成為聲波導藥的介質。然震波而卻可能造成組織損 傷。此外目前仍缺乏探討利用震波經皮導入麻醉藥物之效果的研究，因此本研究目的為 經由震波輔助預先導入及同時導入安麻樂（Eutectic Mixture of Local Anaesthetics, EMLA cream)局部麻醉乳膏的動物實驗，測量體外震波經皮導入麻醉藥物的效果。方法：本兩年期計畫將使用成年之雄性Wistar大鼠為實驗對象。第一年，大鼠將被 隨機分配至以下的組別：對照組、局部塗藥組、超音波導藥組（1MHz)、震波預先導藥組 及震波同時導藥組。除對照組及局部塗藥組外將以不同方法於大鼠尾巴進行EMLA局部 麻醉樂物導樂，每次實驗時間為六十分鐘。過程中以神經傳導研究在每次治療前五分鐘 及治療後一小時内每五分鐘測量所得之感覺神經動作電位的振幅和潛伏時間來評估麻 醉程度。第二年，大鼠將被分配至五個組別，接受不同脈衝數和強度的震波輔助EMLA 局部麻醉藥物導入，來測定此應用的最小劑量。預期結果：此二年期計畫核准執行並順利完成，將可建立此一新型之震波輔助經皮導入 麻醉藥物法。此治療方法不論對於臨床應用或新型醫療儀器發展都極具實用價值。<br> Abstract: Background and Purpose: Traditionally low-frequency ultrasound is used for sonophoresis and has been found to be the most effective method in enhancing transdermal drug transport. The generally accepted mechanism responsible for skin permeability enhancement by sonophoresis might be the cavitation effect. Extracorporeal shockwave is developed to be a potent generator of transient cavitation more than most other forms of medical ultrasound with minimal thermal effect. Thus, it might have better medium than ultrasound for sonophoresis. However, extracorporeal shockwave might induce tissue damage. There is lack of study explored the sonophoretic effect of shockwave on transdermal delivery of anesthetic drug. Therefore, the purposes of this study was to use the determined the sonophoretic effect of extracorporeal shockwave by using the pre- or concurrent shockwave-aided Eutectic mixture of local anesthetics (EMLA cream) application via an animal study.Methods: Adult male Wistar rats will be used as the experimental subject in this two year project. In the first year, the rats will be assigned to following groups randomly: control, topical, sonophoresis-aided (1MHz ultrasound), pre- and concurrent shockwave-aided EMLA application on the rat tails for 60 minutes. The degree of anesthesia was assessed using the amplitude and latency of sensory nerve action potential which were evaluated by nerve conduction study before and every 5 minutes after treatment for 60 min. In the second year, Rats will be divided into five groups to received shockwave mediated EMLA delivery with .different pulse number and intensity to determine the minimal dosage in this application.Expected results: If this two-year project was granted and completed, a novel protocol, shockwave mediated transdermal drug delivery could be established. This protocol is valuable for both clinical application and development of new medical devices.關鍵字：超音波導入體外震波空泡效應感覺神經傳導SonophoresisExtracorporeal shockwaveCavitationSensory nerve conduction