2020-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/701606摘要：生物三維列印(three-dimensional printing, 3DP)為近年來興起的生物支架精準製造技術，主要是將生物墨水材料與細胞混和後進行列印。然而目前生物墨水的選擇極為有限，無法滿足不同組織或器官的需求。本三年期計畫擬開發新一代具有生物可降解、可光交聯調控自癒合之生物墨水材料與可糖解或溫度溶解之犧牲材料，生物墨水透過犧牲材料的輔助，可列印複雜的簍空結構，將更貼近人體的組織或器官。本三年期計畫主要分為三大部分，第一部份為製備具有生物可降解、可光交聯調控自癒合之生物墨水材料與可糖解或溫度溶解之犧牲材料，並分析其基本性質、列印性質與特性產生之原理機制；第二部分為設計、列印仿生組織或器官，並測試幹細胞在墨水材料中之細胞活性與功能性，以及整體組織或器官；第三部分以細胞實驗(例如細胞活性和細胞分化)與動物實驗(斑馬魚血管新生、斑馬魚血管修復及小鼠下肢缺血)驗證此3DP仿生組織或器官的功能性。透過本計畫的執行，期望可製作出更仿生的3DP人造組織或器官，應用於心臟血管系統或其他組織的修復、再生與重建。<br> Abstract: Three-dimensional printing (3DP) is an emerging technology in making precision scaffolds for tissue engineering, where hydrogel as bioink is deposited together with living cells. However, there are only limited options of bioink that still do not meet the demand of different kinds of tissues or organs. In this three-year project, we aim to develop new smart bioink that is biodegradable with printability through self-healing behavior that can be switched on/off and modulated by photoreaction. We will also develop 3D printable and glucose-sensitive and thermosensitive sacrificial materials. We attempt to combine 3D printable bioink and sacrificial materials to fabricate complicated and hollow structures. The tasks of this project include (1) Synthesis and analysis of new type of smart bioink and the development of 3D printable sacrificial materials; (2) Designing biomimetic constructs and printing the bionk along with the sacrificial materials, and evaluating the cytotoxicity and cell viability of stem cells within the biomimetic constructs; and (3) evaluation for biofunctionality of the constructs through cellular experiments (such as gene and protein expression) and animal models (such as Zebrafish blood capillary-forming, Zebrafish heart defect repair, and rat hind limb ischaemia). By the execution of this project, we anticipate to optimize the properties of bioink and the fabrication of biomimetic tissue or organs. Last but not least, the therapeutic potential of the bioink for cardiovascular diseases or other tissue reconstructions can be substantiated by in vivo experiments.生物墨水3D生物列印自癒合材料犧牲材料血管心肌Bioink3D bioprintingself-healing materialssacrificial materialsvascularmyocardium