Lee, Ming-YiMing-YiLeeLin, Huan-YouHuan-YouLinCheng, FaiwenFaiwenChengChiang, Wen-ChangWen-ChangChiangKuo, Yueh-HsiungYueh-HsiungKuo2010-07-152018-06-292010-07-152018-06-292008http://ntur.lib.ntu.edu.tw//handle/246246/189494Five active compounds that inhibit cancer cells were isolated from adlay bran (Coix lachryma-jobi L. var. ma-yuen Stapf), and their structures and activities in vitro were characterized. The ethyl acetate-soluble fraction of methanol extracts of adlay bran (ABM-EtOAc) exhibited a stronger anti-proliferative effect on human lung cancer cell A549, human colorectal carcinoma cell HT-29, and COLO 205 than other fractions by MTT (3-4,5-dimethylthiazol-2-yl-2,5-diphenyl-tetrazolium bromide) assay. Assay-guided isolation gave five lactams including three that were previously undocumented; coixspirolactam A (1), coixspirolactam B (2), and coixspirolactam C (3); one isolated from the natural plant for the first time, coixlactam (4); and one known compound, methyl dioxindole-3-acetate (5). Pure active compounds were identified by spectral analysis including IR, 1H and 13C NMR, UV-vis, MS and 2D NMR techniques. All the compounds were tested for their anti-proliferative effect on A549, HT-29 and COLO 205 cells. These compounds showed anti-cancer activities with IC50 values between 28.6 and 72.6 μg/mL. ? 2008 Elsevier Ltd. All rights reserved.en-USAdlay bran; Anti-proliferative; Colorectal carcinoma; Lactam; Lung cancer[SDGs]SDG33 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; acetic acid ethyl ester; Coix lachryma jobi extract; coixlactam; coixspirolactam a; coixspirolactam b; coixspirolactam c; lactam derivative; methanol; methyl dioxindole 3 acetate; plant extract; antineoplastic activity; article; bran; cancer cell; cell proliferation; Coix; coix lacryma jobi; colorectal carcinoma; controlled study; drug activity; drug isolation; drug structure; human; human cell; lung cancer; medicinal plant; nuclear magnetic resonance spectroscopy; Antineoplastic Agents, Phytogenic; Cell Proliferation; Coix; Colonic Neoplasms; HT29 Cells; Humans; Lactams; Lung Neoplasms; Magnetic Resonance Spectroscopy; Mass Spectrometry; Methanol; Solvents; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Tetrazolium Salts; Thiazoles; Coix lacryma-jobijournal article10.1016/j.fct.2008.01.033http://ntur.lib.ntu.edu.tw/bitstream/246246/189494/1/Isolation and characterization of new lactam compounds that inhibit lung and colon cancer cells from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) bran.pdf