趙福杉臺灣大學:醫學工程學研究所王韻茹Wang, Yun-JuYun-JuWang2010-05-182018-06-292010-05-182018-06-292009U0001-2001200913021800http://ntur.lib.ntu.edu.tw//handle/246246/183697為了研究大腦之記憶、學習及認知等高階功能與大腦神經網路之連結，在動物處於清醒狀態下進行實驗為不可避免之趨勢。對清醒動物進行神經電信號記錄不僅是時代之潮流也是了解大腦功能所必需。本實驗室先前多在麻醉狀態下進行動物實驗，如今正朝著清醒狀態下之電生理記錄前進。因此，本論文為此目標之先期研究。 本論文利用micro-wire電極植入大鼠之大腦體感覺皮質區。在一個月的觀察中，分別在大鼠處於清醒與麻醉狀態下記錄機械性刺激所誘發之神經動作電位。 經由比較兩種狀態下誘發性神經動作電位之信號雜訊比、峰對峰值及延遲時間，結果顯示大鼠處於麻醉狀態時其信號雜訊比與波形峰對峰值明顯比清醒狀態時還大 (p<0.001)，而其延遲時間則無顯著差異 (P>0.05)。經由長期觀察，得知micro-wire電極可穩定記錄特定神經單元。To understand higher functions of the brain such as memory, learning, and cognition and network connection of the brain, electrophysiological recordings in conscious animals are indispensable. Hence, recording neuronal signals in conscious animals is a trend and is the kernel technique to reveal authentic brain function. Animal studies in our laboratory are moving from anesthetic state to conscious model. This thesis is a pilot study for this goal. In this study, evoked action potentials were recorded in the somatosensory cortex (S1) of rats both in conscious and anesthetic states. Homemade micro-wire electrodes were implanted in S1 for one month. Mechanical stimuli were applied to induce neuronal action potentials (APs). Comparing these APs in both states, the signal-to-noise ratio (SNR) and peak-to-peak amplitude in anesthetic state were larger than those in conscious state (p<0.001). The latency of APs shows no significant difference between these states (p>0.05). After long-term observation, it is suggested that neuronal signals can be stably recorded by these homemade micro-wire electrodes.目 錄 試委員會審定書...........................................I謝......................................................II文摘要.................................................III文摘要..................................... ...........IV 錄......................................................V 目錄...................................................VII目錄.................................................VIII 一章 緒論...............................................1 1.1 前言..............................................1.2 研究動機與目的........................................3二章 研究方法與架構.....................................5 2.1 電極設計規格、製作流程與電氣特性之量測............6 2.2 動物手術.........................................10 2.3 實驗步驟與記錄系統...............................12 2.4 冷凍切片.........................................17 2.5 動作電位分析與統計分析..........................18 2.6 電極記錄效能之檢測...............................20三章 實驗結果..........................................21 3.1 micro-wire電極成品..............................21 3.2 信號分析結果....................................23 3.3 電極記錄效能檢測結果............................27四章 討論..............................................31 4.1 峰對峰值.........................................32 4.2 信號雜訊比.......................................33 4.3 延遲時間.........................................34 4.4 電極之記錄效能...................................35五章 參考文獻..........................................36錄一....................................................40application/pdf1606963 bytesapplication/pdfen-US清醒狀態體感覺皮質區觸覺戊巴比妥鹽micro-wire電極conscious statesomatosensory cortextouchsodium pentobarbitalmicro-wire electrodethesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/183697/1/ntu-98-R96548009-1.pdf