Chiang, C.-Y.C.-Y.ChiangHsu, K.-D.K.-D.HsuLin, Y.-Y.Y.-Y.LinHsieh, C.-W.C.-W.HsiehLiu, J.-M.J.-M.LiuLu, T.-Y.T.-Y.LuKUAN-CHEN CHENG2021-06-222021-06-222020https://www.scopus.com/inward/record.url?eid=2-s2.0-85083506245&partnerID=40&md5=cc2a88ac5ee412e333cef2e2bfece713https://scholars.lib.ntu.edu.tw/handle/123456789/566089Androgen-independent prostate cancer accounts for mortality in the world. In this study, various extracts of a medical fungus dubbed Ganoderma formosanum were screened for inhibition of DU145 cells, an androgen-independent prostate cancer cell line. Results demonstrated that both hexane (GF-EH) and butanol (GF-EB) fraction of G. formosanum ethanol extract inhibited DU145 cell viability in a dose-dependent manner. GF-EH induced cell-cycle arrest in G1 phase of DU145 cells via downregulation of cyclin E2 protein expression. In addition, GF-EB triggered extrinsic apoptosis of DU145 cells by activating caspase 3 gene expression resulting in programed cell death. Above all, both GF-EH and GF-EB show lower toxicity to normal human fibroblast cell line compared to DU145 cell, implying that they possess specific drug action on cancer cells. This study provides a molecular basis of G. formosanum extract as a potential ingredient for treatment of androgen-independent prostate cancer. ? 2020, ? 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of the Korean Society of Mycology.apoptosis; cell cycle arrest; cyclin; Ganoderma formosanum; Prostate cancer[SDGs]SDG3journal article10.1080/12298093.2020.17460642-s2.0-85083506245WOS:000526511700001