2009-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/710223摘要：本計畫主旨為延續先前「微尺度多相流場的研究」之群體計畫成果，希望以三年期間發展高效能的創新混合器並應用於生醫流體之混合及檢測、開發流場混合之定性及定量測定技術、輔以平行運算的數值方法模擬分析更詳細的流場結構，希望可以明確定位本研發成果在生化領域與機電領域之Lab on a Chip 應用利基。 研究主要有三大並行的主軸，包含創新之混合元件設計與模擬分析、混合量測技術的開發以及混合機制的理論分析、Lab Chip 於生化上的應用。 創新混合器方面，可有效應用於生醫檢測以及化工合成方面的流體混合。主要發展被動式以及主動式元件兩大類，對於被動式元件而言，利用分離與再結合的概念造成混合，此混合機制包含了大幅度的對流混合及分配作用，突破以往溝槽式混合器增強混合的極限；而主動式混合則是開發增強雜交(hybridization)之元件，利用介面的擾動及作用力加速雜交的效率，可大幅減少傳統上雜交過程所需的時間。過程中將輔以模擬分析，設計最佳化的幾何外型之混合元件，在實驗分析中將發展全面性的混合量測技術，除了流場可視化(螢光、染料的觀測)定性觀測之外，亦利用μ-PIV 與共軛焦顯微鏡做定量上的研究。在混沌混合機制的分析，除了更多週期的流場模擬之外，也採用Poincare 截面以及Lyapunov 指數之演算法去鑑定系統混沌的強度，因此平行運算技術成為流場分析及混合機制探討的一大工具。 預期研究成果將投稿國際學術期刊論文數篇(鎖定Lab Chip, APL 及JMEMS)，包含高效能生醫流體之混合元件的開發、量測技術的全面開發、混沌混合機制的理論分析等；至於專利的產出則涵蓋本研究所有微混合元件的發明。最終期望將應用端推廣至生化方面的Lab-on-a-Chip 檢體混合、燃料電池之電化學混合以及熱流元件之混合效能提升。 <br> Abstract: The objectives of this proposal are to design several novel mixing devices based on principles of chaotic mixing (the mechanisms of split and recombination) and disturbance of interface for enhancement of hybridization, respectively. In-depth microfluidic analysis on flow structure and mixing mechanisms is also conducted via numerical software CFD-RC and experiments using confocal microscope and micro PIV. The results are expected to be extended to the applications of lab-on-a-chip in life science, bio-chemistry, and micro machines. Three Ph.D. students and 1 master students will participate into the sub-programs of (a) the application of mechanisms of split and recombination on mixing and device design (方偉峰), (b) measure techniques of mixing (童凱煬), (c) development of device with enhancing hybridization (楊宗翰), (d) development of theory of chaotic mixing (邱朝陽) and (e) developing sensors and actuators for lab-on-a-chip in applications of bio-chemistry and micro machines. The expected outcomes consists of novel mixing devices for bio-fluidic mixing, new measurement techniques (fluorescence resonance energy transfer (FRET), con-focal microscopy, micro-PIV) for microfluidic mixing, 3-D transient flow structure related with good mixing performance, new mixing theories and mechanisms. The results will be filed for several patents and the academic innovation will be submitted for publications to prestigious journals.微流體混合器雜交生醫檢測mixing devicehybridizationmicromixerconfocal microscopemicro PIV