2021-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/671573"脂締素能促進哺乳動物之脂肪氧化、葡萄糖利用並維持能量的恆定，但脂締素的功能在家禽所知甚少，釐清脂締素影響雞隻的生理、生長及營養代謝功能，有助於瞭解飼糧中的營養分能否藉由脂締素訊息路徑影響雞隻生長或代謝，做為畜牧生產之應用。本團隊前期的計畫已成功建立脂締素在雞隻的基本資訊，包含血液中含量、型態分布及與各血液生化值的相關性。此外，也發現高脂餵飼下會改變雞隻血液中脂締素的含量、並使脂肪組織脂締素受體表現、高分子形式脂締素及二硫氧化還原酶 (為脂締素聚合之重要酵素) 的提升，諸多證據顯示脂締素在雞隻脂質代謝上有重要功能。 本計畫目標將延續前期計畫的成果，著重於探討多元型態的脂締素對雞隻脂質代謝的影響。預計建立雞隻脂肪細胞、肝細胞培養系統，在細胞平台檢驗不同型態的雞隻脂締素對脂質代謝的影響，且利用CRISPR技術剔除脂締素受體，反證其功能；本團隊過去的國際合作成果，發現小鼠的 Translationally Controlled Tumor Protein (TCTP) 能夠調節脂締素訊號並影響肝臟脂質及醣類代謝，但TCTP於雞隻的表現及功能研究尚未明朗，探討APPLs/TCTP/AdipoRs 複合體在雞隻的分子機制亦為本計畫的重點，本計畫將可以瞭解脂締素在雞隻生理及營養的功能，並且將釐清的機制配合營養分調控雞隻脂締素之功能，進一步將結果應用於產業。" "In mammals, adiponectin has been demonstrated to enhance fatty acid oxidation, glucose utilization and improve insulin sensitivity. However, the understanding of adiponectin on physiological and nutritional function in chicken is limited. A better understanding of mechanisms by which nutrients are utilized is essential for achieving optimal animal performance. According to previous study, we have successfully established the expression profiles of adiponectin and its correlation with biochemical variables. Moreover, we found the levels of circulatory adiponectin, high-molecular weight adiponectin and expression of adiponectin receptors and DsbA-L (a multimerization enzyme of adiponectin) can be regulated by high-fat diet feeding. There are some evidences adiponectin has a critical function on lipid metabolism in chicken. The current aim will continue the previous project, focusing on the effects of different forms of adiponectin on lipid metabolism in chicken. We will establish in vitro chicken cell culture systems to elucidate effects of recombinant adiponectin on adiponectin signaling with the use of CRISPR technology to validate potential function of key proteins in the regulation of adiponectin signaling, especially APPLs/TCTP/AdipoRs complex in chicken. Based on the above specific aims and the implementation of the project, we will be able to understand the function of adiponectin signaling in the regulation of physiological and nutritional aspects of chicken growth and development and explore application to the animal production."脂締素脂締素受體雞脂質代謝adiponectinadiponectin receptorschickenlipid metabolism