2010-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/683451摘要：植物在其生活史中常常遭遇許多生物性與非生物性之逆境。乾旱逆境會影響到不同種類不同功能之基因表現。調控基因表現大部分是位於轉錄階層且由轉錄因子所調控。根據Kamiya et al. (2002)之微陣資料，我們挑選了一個由乾旱所誘導的基因，At2g20880來進行研究。At2g20880含有一個高度保守的AP2 domain，屬於DREB亞科。在預備實驗中，我們發現本基因可因乾旱而誘導但不受高溫與ABA之誘導。此外我們也發現過度表達的植物對乾旱的耐受性要比也生的好很多，本研究中我們會是使用各種反式遺傳的研究方法來研究At2g20880的功能。（第一年）我們將研究組織專一性表現，細胞內的位置，突變株與過度表達植株的生理現象。（第二年）我們將利用pull-down與共同免疫沈澱的研究方式發掘任何可與At2g20880發生反應的蛋白質。（第三年）我們將利用微陣的技術來顯示At2g20880過度表達植株過度表現與受抑制的基因。並使用RT-PCR或北方墨點轉漬法去驗證這些表現發生變異的基因。最後我希望對此基因在遭受逆境之下之分子功能獲得徹底的了解。<br> Abstract: Plants often meet many biotic and abiotic stresses through their life span. Assortments of genes with diverse function are influenced by dehydration stresses. Most of their gene products may function in stress responses and tolerance at the cellular level. The predominant mechanism for controlling plant gene expression is regulated at the transcriptional level and is mediated by transcription factors. According to the microarray data of Kamiya et al. (2002), we have selected a dehydration-induced gene, At2g20880 to study. At2g20880 contains one highly conserved AP2 domain and belong to the DREB subfamily. In a preliminary experiment we found that this gene was highly inducible by dehydration but not by high temperature and abscisic acid. In addition, we found overexpressed plant survived better than wild-type under water deficient condition. In this study we will use various reverse-genetic approaches to study the function of At2g20880. (the first year) We will study tissue specific expression, cell localization, the physiology of knock-out and over-expressed plant. (the second year) we will use pull-down and co-immunoprecipitation to study any protein would interact with At2g20880 in vitro and in vivo, respectively. (the third year) We would like to use microarray to reveal the up-regulated and down-regulated gene using At2g2080 over-expressing plants. Using RT-PCR or northern blot validate the expressed genes. Eventually I hope to explicitly understand the molecular function of this gene under stress.阿拉伯芥轉錄子At2g20800乾旱誘導功能性基因体微陣Arabidopsis thalianatranscription factor At2g20800dehydration induciblefunctional genomicsmicroarray.