Hu, C.-W.C.-W.HuTseng, C.-W.C.-W.TsengChien, C.-W.C.-W.ChienHuang, H.-C.H.-C.HuangKu, W.-C.W.-C.KuLee, S.-J.S.-J.LeeChen, Y.-J.Y.-J.ChenJuan, H.-F.H.-F.Juan2018-09-102018-09-102013http://www.scopus.com/inward/record.url?eid=2-s2.0-84883769174&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/378174MicroRNAs (miRNAs) are noncoding RNAs that control gene expression either by degradation of mRNAs or inhibition of protein translation. miR-148a has been reported to have the impacts on tumor progression. Here, a quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins. The data have been deposited to the ProteomeXchange with identifier PXD000190. A total of 2938 proteins were quantified, and 55 proteins were considered to be regulated by miR-148a. We found that not only proteins associated with cancer progression but also molecules involved in neural development were regulated by miR-148a. This study is the first to identify the function of miR-148a in neural development by using a proteomic approach. Analysis of a public clinical database also showed that the patients with neural diseases could display abnormal expression of miR-148a. Moreover, silencing of miR-148a led to the abnormal morphology and decreased expression of neuron-related markers in the developing brain of zebrafish. These results provided important insight into the regulation of neurological development elicited by miR-148a. ? 2013 American Chemical Society.iTRAQ; miR-148a; neurological development; proteome; zebrafish[SDGs]SDG3microRNA; microRNA 148a; stable isotope; unclassified drug; animal model; article; cancer growth; cell line; chromatography; controlled study; DNA degradation; DNA flanking region; embryo; gene control; gene expression; gene function; gene silencing; genetic transfection; human; human cell; in vivo study; morphology; nervous system development; neurologic disease; nonhuman; priority journal; protein synthesis; proteomics; stomach cancer; tumor growth; wild type; zebra fish; Alzheimer Disease; Animals; Base Sequence; Brain; Case-Control Studies; Cell Line, Tumor; Disease Progression; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs; Parkinson Disease; Proteomics; RNA Interference; Sequence Homology, Nucleic Acid; Spinal Cord; Stomach Neoplasms; Transcriptome; Zebrafishjournal article10.1021/pr400302w