2006-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/671280摘要：生醫光電的快速發展，在國內外都是重要的研究課題，在此國際雙邊合作計畫中，我們將與法國卡相高等師範學院(ENS Cachan)的LPQM實驗室Prof. Sophie Brasselet及Prof. Zyss進行非線性光學顯微術於分子系統動態行為量測解析之研究，配合雙方具有互補性之現有非線性光學系統與研究專長，共同進行在奈米與微米等級的生物分子系統(DNA, Proteins)的結構、排列與交互作用等靜態與動態訊號的量測與分析。因其點激發的特性，相較於傳統光學顯微鏡有許多優勢，如對生物樣本的損害較微弱、穿透深度較深，因此適合用來觀察活體生物樣本，也可透過對光場強度、波長與極化狀態的選擇，達到對分子狀態的分析與控制。本研究計畫將分三年進行，逐年由較簡單的分子薄膜模型，進入到單細胞與組織的量測與應用。包括 1. 改進現有雙光子顯微系統之功能使之具有更先進之倍頻與動態檢測以提供互補而相互支援的功能 2. 研究具有電化學活性之介面層自組裝分子之長度與共軛官能基對其電子共振狀態之影響，以應用於微流道表面特性之操控與感測 3. 研究類似液晶分子如膽固醇於細胞膜組裝中在電場影響下的分佈操控情形.<br> Abstract: Biomedical optics has been an emerging topic of recent research interests. In this bi-lateral collaboration project, we will team up with Prof. Sophie Brasselet and Prof. Joseph Zyss of LPQM, ENS Cachan, France, to address the issues in observing and analyzing dynamics of biomolecule in Micro/Nano domains. We will coordinate our current efforts on existing and yet complement to each other of two nonlinear microscopic systems in LPQM and CNST, NTU, to investigate the static/dynamic changes of optical signal due to structure or interaction of biomolecules, e.g., DNA or protein. Due to its optical characteristics of deeper peneration and less damage to the tissue with longer wavelength, it is preferable for in vivo observation than the traditional optical methods. With optical design of various configurations, one can use field intensity, wavelength, and/or polarization to detect the target molecule and to manipulate it with local effect. In this project proposal, we will work on common interests of optical properties of organic thin film, cellular membrane, single cell and cell/tissue in three years. These include 1. Further integration of SHG and FCS capabilities of our custom made TPM system to provide complement and yet complete features for the first year. 2. Looking into the functional surface molecules, with electrical active function group, e.g. Fc+Alkanthiol, for the effect of total length and the existance of electron acceptor/donor of pi-electron or co-polymer for electron resonance manipulation to serve as possible means for electrical control of surface properties in microfluidic application. 3. Liquid crystal like molecule of Cholesterol embbed in bilipid membrane with potetnial mapping/manipulation. In vitro/In vivo appliacitons of above developed techniques for cell cultures as potetnial cell chip applications.生醫光電雙光子顯微術倍頻顯微術表面電漿共振Biomedical opticsTwo photon microscopySceond Harmonic Generation MicroscopyPlamonics.