Lin, Hsin-HungHsin-HungLinWang, Chih-YenChih-YenWangHsieh, Feng-JenFeng-JenHsiehLiao, Fang-ZhenFang-ZhenLiaoSu, Yu-KaiYu-KaiSuPham, Minh DinhMinh DinhPhamCHIH-YUAN LEEChang, Huan-ChengHuan-ChengChangHSAO-HSUN HSU2023-06-302023-06-302023-02-0715565068https://scholars.lib.ntu.edu.tw/handle/123456789/633265The use of nanodiamonds (NDs) as biocompatible non-allergic additives in incomplete Freund’s adjuvant (IFA) to elicit immune responses is investigated. With chicken egg ovalbumin (OVA) as model antigens, experiments of mice immunized with OVA and OVA/ND in IFA showed that this new formulation could significantly enhance the immune responses and reduce the consumption of antigens to produce the antibodies of interest. It serves well as a safe and effective vaccine adjuvant without the need of inactivated mycobacteria pathogens that could cause many allergic reactions. In addition, the composites exhibited distinct therapeutic activities, as proven by the OVA/ND/IFA treatment which could effectively inhibit the tumor progression of OVA-expressing E.G7 cells inoculated in mice over 35 days. About 0.2% of the injected ND particles were found in mouse spleens on day 24. The multiple functionality of ND makes it useful as an active and trackable component of a vaccine adjuvant. © 2023, The Authors. All rights reserved.enAdjuvants; antitumor agents; fluorescent nanodiamonds; ovalbumin; vaccineother10.2139/ssrn.4334118