SHI-MING LINCHIH-KUNG LEELee S.-Y.CHUAN-LIANG KAOCHII-WANN LINAN-BANG WANGHsu S.-M.Huang L.-S.2009-01-212018-06-292009-01-212018-06-292005http://ntur.lib.ntu.edu.tw//handle/246246/107061http://ntur.lib.ntu.edu.tw/bitstream/246246/107061/1/16.pdfhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-28044445658&doi=10.1111%2fj.1462-5822.2005.00593.x&partnerID=40&md5=9634732d574da92007ee988573046ccaAtomic force microscopy has been used to probe the surface nanostructures of severe acute respiratory syndrome coronavirus (SARS-CoV). Single crown-like virion was directly visualized and quantitative measurements of the dimensions for the structural proteins were provided. A corona of large, distinctive spikes in the envelope was measured after treatment with hydroxyoctanoic acid. High-resolution images revealed that the surface of each single SARS-CoV was surrounded with at least 15 spherical spikes having a diameter of 7.29 ± 0.73 nm, which is in close agreement with that of S glycoproteins earlier predicted through the genomes of SARS-CoV. This study represents the first direct characterization of the surface ultrastructures of SARS-CoV particles at the nanometre scale and offers new prospects for mapping viral surface properties. © 2005 Blackwell Publishing Ltd.application/pdf662181 bytesapplication/pdfen-USjournal article10.1111/j.1462-5822.2005.00593.x163094622-s2.0-28044445658http://ntur.lib.ntu.edu.tw/bitstream/246246/107061/1/16.pdf