2014-01-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/658424摘要：每年因癌症而死亡的人數在台灣及其它先進國家中，仍是高居於榜首,且遠高於其他疾病。但若能提高癌症初期診斷之發現，將可顯著提升95%之癌症存活率。因此，近年來發展針對提高癌症初期診斷，而須具備下列要件之影像系統，成為當今格外重要的研究課題。(1)不需掃描，可即時(real time)取得三維影像及光譜訊息。(2)高解析度。(3)可取得生物組織之空間結構與光譜資訊。(4)低成本。(5)具備可調變的影像視野(field of view)。光學影像系統在生醫影像技術中，具備上述大部份的條件。與其他影像系統相比，光學影像系統可提供毫米與微米的高解析度，遠超過一些臨床上常用的超音波(ultrasound)技術。另外，就系統的複雜度與成本而言，與磁振攝影(MRI)或電腦斷層 (CT)相比較，光學影像系統更具優勢。目前常用的光學生醫影像系統，像共軛焦顯微鏡(confocal microscopy)及光學同步斷層攝影(optical coherence tomography)，解析度明顯優於其他非光學的影像系統(包括:超音波、MRI與CT)，但是穿透深度則較差。雖然光的穿透力，無法像核磁共振或電腦斷層那麼深，但確足夠看到多數癌症病變起源於器官組織的表層，提供癌症初期診斷的關鍵資訊。理論上，光學影像系統可以提供所測試生物組織的三維影像。但目前所用的光學生醫影像系統，均須要透過複雜的掃描設計(包括:橫向或縱向掃描)來完成。這些複雜的掃描設計不僅增加設計成本，並大幅降低穩定度。另外，耗時且增加術後清潔的難度。目前，尚無任何一種三維影像系統可以在不掃描的情形下，同時且即時取得活體生物組織的三維空間結構與光譜資訊。「三維空間暨光譜多工全像顯微影像系統」是新型光學醫學影像系統，可同時取得生物組織的三度空間訊息(x,y,z)並同時獲得其光譜(λ)特性，並且不需要透過複雜的掃描機制，可達到即時影像的功能。本系統將運用多工體積全像布拉格光柵( multiplexed volume holographic Bragg gratings)作為空間及光譜濾波器，搭配傳統的光學元件，達到其他傳統光學生醫影像系統所沒有的優點，包括:同時具備高空間、高光譜解析度、成本相對低廉、且不需掃描就可取得三維空間與光譜的資訊。相信「三維空間暨光譜多工全像顯微影像系統」將對癌症發生之研究、組織病變，甚至其他醫學用途具有卓越的貢獻。<br> Abstract: Cancer is the leading cause of death in many countries, as well as in Taiwan. The five-year survival rate is improved to 95% if the cancer is discovered at an early, localized stage. Therefore, much effort has been expended in recent years developing imaging methods and instrumentation for early detection. A desired imaging modality would be:1.three-dimensional (3D) imaging with minimal or no scanning 2.high resolution 3.sensitive to structure and to biochemistry 4.simple and low-cost 5.equipped with a variable field of view. Optical imaging systems possess many of these desirable characteristics. Their resolution may reach submicron levels, far exceeding other clinical imaging modalities such as ultrasound. The cost and complexity of optical systems is low compared to modalities such as magnetic resonance imaging or computed tomography. Optical systems suffer from an inherently small imaging depth in scattering tissue. However, many optical imaging modalities have sufficient large depth of imaging to visualize the epithelial layer (genesis of many cancers) of the skin (directly) or various organ tissues (through rigid or flexible endoscopes).Ideally, optical imaging systems would provide 3D images, enabling characterization of a volume of tissue. However, all these systems have complex mechanical designs, requiring scanning in two lateral dimensions, or depth focusing. The complex designs increase device cost, reduce robustness, and increase the complexity of meeting patient interface/sterilization issues. To our knowledge, no three-dimensional combined structural and biochemical imaging system suitable for in vivo biomedical imaging without scanning has been developed.The overall goal of the proposed research is to develop and test a novel optical imaging system that requires no scanning and can extract simultaneous spatial and spectral information from biological tissue samples. The proposed system is based on volume holographic Bragg filters used in conjunction with conventional optical imaging components to form a multiplexed volume holographic imaging (MVHI) system, which has many advantages over competing optical imaging techniques for biological tissue samples. These include simultaneous high spatial and spectral image resolution with the same optical system, use of inexpensive diode laser illumination, and elimination of scanning parts. This is a revolutionary advance that we believe will open the way for better understanding of the onset of cancer and biological tissue morphology as well as other clinical uses through real time observation capability.三維影像光譜資訊全像顯微術多工3D spatial imagingspectral informationholographymicroscopymultiplexing