Tseng, C.-H.C.-H.TsengKnowles, N.J.N.J.KnowlesHSIANG-JUNG TSAI2018-09-102018-09-102007http://www.scopus.com/inward/record.url?eid=2-s2.0-33846153230&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/332419The genome sequences of three duck hepatitis virus type 1 (DHV-1) strains were determined. Comparative sequence analyses showed that they possessed a typical picornavirus genome organization apart from the unique possession of three in-tandem 2A genes. The 2A1 protein of DHV-1 is an aphthovirus-like 2A protein; the 2A2 protein is not related to any known picornavirus protein; the 2A3 protein is a human parechovirus-like 2A protein. Several other features were found to be unique to the DHV-1 genome when compared with other picornaviruses: (i) the 3′ UTR of DHV-1 was composed of 314 nt, the largest among the picornaviruses; (ii) pair-wise amino acid sequence identities between polyprotein of DHV-1 and other picornaviruses are all less than 30%. The pair-wise amino acid sequence identities in the 3D region of DHV-1 with LV and HPeV-1 is only 38.6 and 36.6%, respectively, and less than 30% with all other picornaviruses; (iii) the DHV-1 capsid polypeptide VP0 is not proteolytically cleaved into VP4 and VP2; and (iv) phylogenetic and evolutionary analysis of DHV-1 reveals a new picornavirus clade. It is therefore proposed that DHV-1 should be assigned to a new genus in the Picornaviridae. ? 2006 Elsevier B.V. All rights reserved.2A protein; DHV-1; Duck hepatitis virus type 1; Picornaviridae[SDGs]SDG3polypeptide; protein 2a; protein 2a3; unclassified drug; virus protein; 3' untranslated region; amino acid sequence; animal cell; article; controlled study; duck hepatitis virus type 1; gene sequence; genetic organization; hepatitis virus; molecular evolution; nonhuman; nucleotide sequence; phylogeny; Picornavirus; priority journal; protein degradation; sequence analysis; sequence homology; viral genetics; virus capsid; virus genome; 3' Untranslated Regions; Americas; Amino Acid Sequence; Capsid Proteins; Cysteine Endopeptidases; Genome, Viral; Great Britain; Hepatitis Virus, Duck; Molecular Sequence Data; Nucleic Acid Conformation; Phylogeny; RNA, Viral; Sequence Alignment; Sequence Homology, Amino Acid; Species Specificity; Taiwan; Viral Proteins; Aphthovirus; Duck hepatitis B virus; Human parechovirus; Miridae; Picornaviridaejournal article10.1016/j.virusres.2006.09.007