Chang, Ming-HuangMing-HuangChangChou, Chih-MingChih-MingChouHsieh, Yueh-ChunYueh-ChunHsiehLu, I-ChingI-ChingLuM.Kothai Nachiar DeviChang, Juei-PeiJuei-PeiChangKuo, Tzong-FuTzong-FuKuoHuang, Chang-JenChang-JenHuang2009-07-292018-07-062009-07-292018-07-062004http://ntur.lib.ntu.edu.tw//handle/246246/162976http://ntur.lib.ntu.edu.tw/bitstream/246246/162976/1/19.pdfThe genomic structure of Tetraodon fluviatilis L29 gene was determined and its promoter activity was analyzed in COS-1 cells and zebrafish embryos. The TfL29 gene comprises four exons and three introns, spanning approximately 1.7kb. The 5′-upstream 2.2-kb of the first exon contains 10 E-boxes and many putative binding motifs for transcription factors GATA-1, AML-1a, c-Myb, Oct-1, CdxA, and NRF-2. Promoter activity assay showed that the distal 2.2-kb fragment not only had high luciferase activity in COS-1 cells, but also strong and ubiquitous GFP expression in a variety of tissues in zebrafish embryos. On the other hand, there are no TATA or CAAT boxes within a 300-bp region upstream from the transcription initiation site. Although this region has high luciferase activity in COS-1 cells, it is not sufficient to drive GFP expression in zebrafish embryos. In this proximal 300-bp region, there are two E-boxes, two CdxA sites, and one NRF-2 site that is immediately downstream of the transcription start site. ? 2003 Elsevier Inc. All rights reserved.application/pdf578539 bytesapplication/pdfen-USprotein c Myb; ribosome protein; TATA binding protein; transcription factor; transcription factor GATA 1; transcription factor RUNX1; green fluorescent protein; hybrid protein; photoprotein; Rpl29 protein, mouse; animal cell; article; cell strain COS1; controlled study; enzyme activity; exon; fish genetics; gene expression regulation; gene identification; gene location; gene structure; genome analysis; intron; nonhuman; nucleotide sequence; priority journal; promoter region; protein expression; protein motif; puffer fish; transcription initiation site; zebra fish; amino acid sequence; animal; Caenorhabditis elegans; chemistry; comparative study; Drosophila; genetics; human; metabolism; molecular genetics; sequence alignment; sequence analysis; sequence homology; species difference; structure activity relation; Tetraodontiformes; transactivation; Danio rerio; Tetraodon; Tetraodon fluviatilis; Tetraodon fluviatilis; Tetraodontidae; Amino Acid Sequence; Animals; Base Sequence; Caenorhabditis elegans; COS Cells; Drosophila; Gene Expression Regulation; Green Fluorescent Proteins; Humans; Luminescent Proteins; Molecular Sequence Data; Promoter Regions (Genetics); Recombinant Fusion Proteins; Ribosomal Proteins; Sequence Alignment; Sequence Analysis, Protein; Sequence Homology, Amino Acid; Species Specificity; Structure-Activity Relationship; Tetraodontiformes; Trans-Activation (Genetics); Zebrafishjournal article10.1016/j.bbrc.2003.12.080http://ntur.lib.ntu.edu.tw/bitstream/246246/162976/1/19.pdf