2015-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/710095Due to the complexity of the root canal system, when it is infected with microbes, the bacteria could invade the slits, lateral canals and ramifications, the bacterial could even penetrate the dentinal tubes to infect the tissues around the root apex. However, it is difficult to clean effectively the root canal system by mechanical debridement or chemical rinse. The key factor for the failure of root canal treatment is not to eliminate thoroughly the bacteria in the dentinal tubes and supplemental canals. Therefore, it is necessary to develop a bioactive material with the ionic permeability to occlude the dentinal tubes and lateral canals, to heal over the root apex with its antibacterial ability. The goals of this three‐year research plan are to develop bioactive and antibacterial materials for the treatment of root canal, to clarify the mechanism of precipitation and crystallization that ions permeate the dentinal tubes, to assess the ability of these materials to occlude the lateral and supplemental canals. The first year is the study of mechanisms: 1) to analyze the physics and chemical properties of DP‐bioglass and mesoporous calcium silicate biomaterials that we have already developed before, to integrate the above‐mentioned materials with Epigallocatechin‐3‐Gallate (EGCG) and Chlorhexidine(CHX), and assess its antibacterial ability. 2) to establish the ionic permeability patterns of dentin shield, analyze the precipitation and crystallization mechanism of ions, and the influences of micro‐crystallization to the tooth structure. The second year: to use the above‐mentioned patterns to study their influences to the mineralization ability of osteoblasts, investigate the reciprocation between the periodontal membrane and the materials, analyze the biocompatibility of the materials, find out the appropriate groups, then proceed the animal testing of the third year. The third year: to establish the animal testing model, evaluation of the reactions to tissues after using the developed bioactive materials to the in vivo root canal treatment.DP‐bioglassmesoporous calcium silicate biomaterialroot canal treatmentlateral canalramificationEpigallocatechin‐3‐Gallate (EGCG)Chlorhexidine (CHX)