Soundararajan RHsu T.-WQin YSHENG-LUNG HUANG2022-04-252022-04-2520221864063Xhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85118171236&doi=10.1002%2fjbio.202100249&partnerID=40&md5=e08b9e713b60486141e1149dd73e6621https://scholars.lib.ntu.edu.tw/handle/123456789/607282With homemade active crystalline fibers, we generated bright and broadband light sources for full-field optical coherence tomography, offering deep penetration into skin tissues with cellular resolution at a high frame rate. Extraction of backscattered spectra from the tissue has potential applications in biomedicine. The hysteresis nonlinearity of the piezoelectric transducer actuating the Mirau interferometer has been greatly reduced by a feedforward compensation approach. The linearized hysteresis response enables us to extract depth-dependent spectra accurately. To validate, the complex dispersion of a fused silica plate was characterized with 2% error. Further validation on an in vitro setting, the backscattered spectra from indocyanine green pigment and nonpigmented microspheres were obtained and verified. For in vivo skin measurement, the backscattered spectra show depth-dependent spectral shift and bandwidth variation due to the complex skin anatomy and pigment absorption. Such a high-speed spectra acquisition of in vivo deep tissue backscattering could lead to disease diagnosis in clinical settings. ? 2021 Wiley-VCH GmbH.Absorption spectroscopyBackscatteringDiagnosisExtractionFused silicaHysteresisLight sourcesOptical data processingTissueBack-scatteredBackscattering spectraDepth dependentsFull-field optical coherence tomographiesImages processingIn-vivoIn-vivo human skinSpectra'sTomographic image processingTomographic imagesOptical tomographydiagnostic imaginghumanoptical coherence tomographyskinHumansSkinTomography, Optical Coherence[SDGs]SDG3journal article10.1002/jbio.202100249346625102-s2.0-85118171236