Tzeng, Yi-ShiuanYi-ShiuanTzengLi, HungHungLiKang, Yuan-LinYuan-LinKangChen, Wen-ChengWen-ChengChenCheng, Wei-ChengWei-ChengChengDAR-MING LAI2023-09-042023-09-042011-01-1300064971https://scholars.lib.ntu.edu.tw/handle/123456789/634997The C-X-C-type chemokine Cxcl12, also known as stromal cell-derived factor-1, plays a critical role in hematopoiesis during fetal development. However, the functional requirement of Cxcl12 in the adult hematopoietic stem/progenitor cell (HSPC) regulation was still unclear. In this report, we developed a murine Cxcl12 conditional deletion model in which the target gene can be deleted at the adult stage. We found that loss of stroma-secreted Cxcl12 in the adult led to expansion of the HSPC population as well as a reduction in long-term quiescent stem cells. In Cxcl12-deficient bone marrow, HSPCs were absent along the endosteal surface, and blood cell regeneration occurred predominantly in the perisinusoidal space after 5-fluorouracil myelosuppression challenge. Our results indicate that Cxcl12 is required for HSPC homeostasis regulation and is an important factor for osteoblastic niche organization in adult stage bone marrow.enjournal article10.1182/blood-2010-01-266833208339812-s2.0-78751559016https://api.elsevier.com/content/abstract/scopus_id/78751559016