Weng, Rui CianRui CianWengTsou, Min ChengMin ChengTsouLee, Jyun LinJyun LinLeeTseng, Chao MingChao MingTsengHuang, Yu FenYu FenHuangXiao, Yu LinYu LinXiaoLu, Yen PeiYen PeiLuChou, Wei ChunWei ChunChouRUEY-FENG CHANGChuang, Chun YuChun YuChuang2024-03-072024-03-072024-05-1500399140https://scholars.lib.ntu.edu.tw/handle/123456789/640561Glyphosate (GLY) is a widely used herbicide worldwide, particularly in cultivating genetically modified soybeans resistant to GLY. However, routine multi-residue analysis does not include GLY due to the complexity of soybean matrix components that can interfere with the analysis. This study presented the development of an aptamer-based chemiluminescence (Apt-CL) sensor for rapidly screening GLY pesticide residue in soybeans. The GLY-binding aptamer (GBA) was developed to bind to GLY specifically, and the remaining unbound aptamers were adsorbed onto gold nanoparticles (AuNPs). The signal was in the form of luminol-H2O2 emission, catalyzed by the aggregation of AuNPs in a chemiluminescent reaction arising from the GLY-GBA complex. The outcomes demonstrated a robust linear relationship between the CL intensity of GLY-GBA and the GLY concentration. In the specificity test of the GBA, only GLY and Profenofos were distinguished among the fifteen tested pesticides. Furthermore, the Apt-CL sensor was conducted to determine GLY residue in organic soybeans immersed in GLY as a real sample, and an optimal linear concentration range for detection after extraction was found to be between 0.001 and 10 mg/L. The Apt-CL sensor exploits the feasibility of real-time pesticide screening in food safety.enAptamer | AuNPs | Chemiluminescence | Glyphosate | Soybean[SDGs]SDG3journal article10.1016/j.talanta.2024.125741383597182-s2.0-85185194485https://api.elsevier.com/content/abstract/scopus_id/85185194485