臺灣大學: 應用力學研究所趙聖德沈佑儒Shen, You-RuYou-RuShen2013-03-212018-06-292013-03-212018-06-292012http://ntur.lib.ntu.edu.tw//handle/246246/249695最近二十年來，微流體裝置如混合器、微幫浦、生物感測器等被廣泛的研究與發展，上述皆為利用力學機制達成混合、輸送、檢測等目的。生物感測器因具備了高靈敏性、微量檢測和即時檢測等特性，提供了即時且便利的生物檢測方式。 在微系統中，交流電動力（ACEK）為一種可操控懸浮顆粒在流體中運動或是擾動流體運動的機制，包括了介電泳（dielectrocphoresis）、交流電滲（AC electroosmosis）及交流電熱力（AC electrothermal）等，而這些交流電動力則會因為懸浮物的帶電量、電解液的導電度及交流電場之電壓有所區分。透過交流電場的施加，可有效操控流場內的懸浮顆粒，或是使流場產生渦流以利攪拌、輸送等工作。 本研究主要探討除了交流電場外另施加一直流偏壓後，所引致之局部導電度的差異造成流場產生一強勁且單一之渦流，將其應用在生物感測器上之免疫分析。利用有限元素分析軟體Comsol Multiphysics對直流偏壓交流電動力應用在生物感測器上討論不同的參數改變以改善其效能。In the last two decades, microfluidic devices such as micromixers, micropumps, and biosensors are popularly investigated and developed. All list above are primarily employed for mixing, transporting, and detecting by means of externally or internally mechanical mechanisms. A biosensor has several advantages such as highly sensitive, tiny mass load detection and capable of monitoring dynamic biomolecular interaction in real time, to be used as the apparatus for the biomolecule detection. AC electrokinetics is concerned with the study of flow stirring, movement and behavior of particles in suspension when they are subjected to AC electrical fields. When microfluidic devices of the microfabricated electrode systems are subjected to AC electrical fields, the AC electrokinetics involving the dielectrophoresis, AC electroosmosis, and ac electrothermal force will be generated according to charges of colloids, different properties of electrolytes or amplitude and frequency of external applied voltages. The thesis presents a numerical study of DC-biased AC-electrokinetic (DC-biased ACEK) flow over a pair of symmetrical electrodes generated by a transverse conductivity gradient occurring at the electrodes when a DC-bias is applied, and use this mechanism on the biosensors. We use the finite element analysis software Comsol Multiphysics for DC-biased AC- electrokinetics applying on biosensors by means of several parameters charging to enhance the binding efficiency of immunoassay.6658339 bytesapplication/pdfen-US免疫分析生物感測器交流電動力直流偏壓交流電動有限元素法immunoassaybiosensorACEKDC-biased AC-electrokeneticsfinite element methodthesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/249695/1/ntu-101-R98543045-1.pdf