醫學工程學研究所Wang, K.K.WangLiu, T.-M.T.-M.LiuWu, J.J.WuHorton, N. G.N. G.HortonLin, C. P.C. P.LinXu, C.C.Xu2013-12-172018-06-292013-12-172018-06-292012-09http://ntur.lib.ntu.edu.tw//handle/246246/258615We demonstrate a fiber-based, three-color femtosecond source for simultaneous imaging of three fluorescent proteins (FPs) using two-photon fluorescence microscopy (2PM). The three excitation wavelengths at 775 nm, 864 nm and 950 nm, are obtained through second harmonic generation (SHG) of the 1550-nm pump laser and the 1728-nm and 1900-nm solitons generated through soliton self-frequency shift (SSFS) in a large-mode-area (LMA) fiber. These energetic pulses are well matched to the two-photon excitation peaks of red, cyan and yellow fluorescent proteins (TagRFPs, TagCFPs, and TagYFPs) for efficient excitation. We demonstrate simultaneous 2PM of human melanoma cells expressing a "rainbow" combination of these three fluorescent proteins.2784398 bytesapplication/pdfen-USNonlinear opticsfibersFluorescence microscopyNonlinear microscopyHarmonic generation and mixing10.1364/BOE.3.001972http://ntur.lib.ntu.edu.tw/bitstream/246246/258615/1/BOE(2012)_three color fs source.pdf