Chang I.-F.Curran A.Woolsey R.Quilici D.Cushman J.C.Mittler R.Harmon A.Harper J.F.2019-07-172019-07-17200916159853https://scholars.lib.ntu.edu.tw/handle/123456789/414137In eukaryotes, 14-3-3 dimers regulate hundreds of functionally diverse proteins (clients), typically in phosphorylation-dependent interactions. To uncover new clients, 14-3-3 omega (At1g78300) from Arabidopsis was engineered with a "tandem affinity purification" tag and expressed in transgenic plants. Purified complexes were analyzed by tandem MS. Results indicate that 14-3-3 omega can dimerize with at least 10 of the 12 14-3-3 isoforms expressed in Arabidopsis. The identification here of 121 putative clients provides support for in vivo 14-3-3 interactions with a diverse array of proteins, including those involved in: (i) Ion transport, such as a K + channel (GORK), a Cl - channel (CLCg), Ca 2+ channels belonging to the glutamate receptor family (1.2, 2.1, 2.9, 3.4, 3.7); (ii) hormone signaling, such as ACC synthase (isoforms ACS-6, -7 and -8 involved in ethylene synthesis) and the brassinolide receptors BRI1 and BAK1; (iii) transcription, such as 7 WRKY family transcription factors; (iv) metabolism, such as phosphoenol pyruvate carboxylase; and (v) lipid signaling, such as phospholipase D (£] and £^). More than 80% (101) of these putative clients represent previously unidentified 14-3-3 interactors. These results raise the number of putative 14-3-3 clients identified in plants to over 300. ? 2009 Wiley-VCH Verlag GmbH & Co. KGaA.14-3-3ArabidopsisInteractomeKinaseTandem affinity purificationjournal article10.1002/pmic.2008004452-s2.0-67649235671https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649235671&doi=10.1002%2fpmic.200800445&partnerID=40&md5=6136086fea052405691488a525e9e06c