Lin DHsieh C.-LHsu K.-CLiao P.-HQiu SGong TYong K.-TFeng SKIEN-VOON KONG2021-08-032021-08-03202120411723https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107214703&doi=10.1038%2fs41467-021-23789-3&partnerID=40&md5=1bfe67fc1133ae807c4671c1653b205chttps://scholars.lib.ntu.edu.tw/handle/123456789/575793The limited availability of nasopharyngeal carcinoma-related progression biomarker array kits that offer physicians comprehensive information is disadvantageous for monitoring cancer progression. To develop a biomarker array kit, systematic identification and differentiation of a large number of distinct molecular surface-enhanced Raman scattering (SERS) reporters with high spectral temporal resolution is a major challenge. To address this unmet need, we use the chemistry of metal carbonyls to construct a series of unique SERS reporters with the potential to provide logical and highly multiplex information during testing. In this study, we report that geometric control over metal carbonyls on nanotags can produce 14 distinct barcodes that can be decoded unambiguously using commercial Raman spectroscopy. These metal carbonyl nanobarcodes are tested on human blood samples and show strong sensitivity (0.07 ng/mL limit of detection, average CV of 6.1% and >92% degree of recovery) and multiplexing capabilities for MMPs. ? 2021, The Author(s).biomarker; blood; cancer; carbonyl compound; differentiation; geometry; molecular analysis; scattering; matrix metalloproteinase; metal nanoparticle; organometallic compound; tumor marker; blood; chemistry; disease exacerbation; genetic procedures; nasopharynx carcinoma; nasopharynx tumor; pathology; procedures; Raman spectrometry; sensitivity and specificity; surface property; Biomarkers, Tumor; Biosensing Techniques; Disease Progression; Matrix Metalloproteinases; Metal Nanoparticles; Nanogels; Nasopharyngeal Carcinoma; Nasopharyngeal Neoplasms; Organometallic Compounds; Sensitivity and Specificity; Spectrum Analysis, Raman; Surface Properties[SDGs]SDG3avidin; biological marker; biotin; carbonyl derivative; collagen type 10; collagen type 11; collagen type 4; collagen type 9; cysteine; gelatin; gelatinase A; gelatinase B; gold nanoparticle; interstitial collagenase; iodine; matrilysin; matrix metalloproteinase; molybdenum; nanogel; rhenium; ruthenium; stromelysin; thiol group; transition element; tungsten; matrix metalloproteinase; metal nanoparticle; organometallic compound; tumor marker; biomarker; blood; cancer; carbonyl compound; differentiation; geometry; molecular analysis; scattering; Article; blood sampling; cancer growth; electromagnetic radiation; Epstein Barr virus infection; genetic susceptibility; human; information science; limit of detection; metastasis; nasopharynx carcinoma; polarization; protein degradation; protein expression; surface enhanced Raman spectroscopy; synthesis; tumor invasion; blood; chemistry; disease exacerbation; genetic procedures; nasopharynx carcinoma; nasopharynx tumor; pathology; procedures; Raman spectrometry; sensitivity and specificity; surface property; Biomarkers, Tumor; Biosensing Techniques; Disease Progression; Matrix Metalloproteinases; Metal Nanoparticles; Nanogels; Nasopharyngeal Carcinoma; Nasopharyngeal Neoplasms; Organometallic Compounds; Sensitivity and Specificity; Spectrum Analysis, Raman; Surface Propertiesjournal article10.1038/s41467-021-23789-3340788952-s2.0-85107214703