Wang W.-K.Lin S.-R.Lee C.-M.King C.-C.SHAN-CHWEN CHANG2020-12-302020-12-3020020022-538Xhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0036229533&doi=10.1128%2fJVI.76.9.4662-4665.2002&partnerID=40&md5=afd3fd5264d5dd0cc8111ecd745a2318https://scholars.lib.ntu.edu.tw/handle/123456789/536233Using reverse transcription-PCR and clonal sequencing of the dengue virus envelope gene derived from the plasma samples of six patients, we reported for the first time that dengue virus circulates as a population of closely related genomes. The extent of sequence diversity varied among patients, with the mean pairwise proportions of difference ranging from 0.21 to 1.67%. Genome-defective viruses were found in 5.8% of the total number of clones analyzed. Our findings on the quasispecies nature of dengue virus and the defective virus in vivo have implications with regard to the pathogenesis of dengue virus.[SDGs]SDG3article; controlled study; Dengue virus; envelope gene; gene sequence; genetic variability; human; molecular cloning; nonhuman; nucleotide sequence; plasma; priority journal; reverse transcription polymerase chain reaction; sequence analysis; virus envelope; virus genome; virus pathogenesis; Amino Acid Sequence; Dengue; Dengue Virus; Genome, Viral; Humans; Molecular Sequence Data; RNA, Viral; Sequence Analysis, DNA; Variation (Genetics); Viral Envelope Proteinsjournal article10.1128/JVI.76.9.4662-4665.200211932434