以代謝體學探討臺灣紅藜調節免疫之功效評估及其活性成分

2016-01-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/711690摘要：臺灣紅藜是藜麥屬的一種臺灣本土傳統植物，亦為原住民的食物來源之一。紅藜與藜麥相似，為富含高蛋白質之高營養作物，其鮮紅色是來自於甜菜色素。紅藜具有許多生理活性，例如抗氧化力、消炎、免疫調節等，然而紅藜的科學報告較少，本計畫參考藜麥在科學上的證據作為旁證。藜麥為低麩質之食材，不易引起食物過敏，且其富含的皂素具有調節過敏免疫之功效，因此紅藜是否具有調節過敏免疫反應是值得研究的。再者，紅藜與藜麥、莧菜籽同屬莧科植物，其脫殼後外觀難以辨別，因此探討紅藜中的化學組成作為紅藜樣品的標準化是必需的。因此，本計畫將以代謝體學的方式探討紅藜其調節免疫之功效及其主要的活性成分。第一年將於細胞模式中探討紅藜在調節免疫反應之活性，並以分離純化的方式找出其活性成分。第二年則是針對紅藜中的化學組成，以液相層析串聯質譜儀進行指紋圖譜的建立，並利用代謝體學的研究方式，採化學計量的多變量統計分析方法，區別出紅藜、藜麥及莧菜籽三者。第三年則是利用標準化後的紅藜樣品進行動物實驗，依據健康食品評估方法來確定紅藜在生物體內的免疫調節活性；此外，利用28 天毒性試驗可評估出紅藜的安全食用劑量，作為未來推廣紅藜之科學依據。<br> Abstract: Djulis (Chenopodium formosanum) is a traditional cereal plant as food source used by Taiwan indigenous people. Djulis, similar to the quinoa (Chenopodium quinoa), is a highly nutrition food with the high content of protein, and its color is come from betalains. Djulis play as a functional food, such as antioxidant, anti-inflammatory and immune-regulatory effect. However, there has less literature focus on the Djulis. This project was depends on the scientific report of quinoa as a circumstantial evidence. Quinoa is recommended as a gluten-free diet, has less food allergen. The saponins from quinoa have immune-regulatory effects in vitro. Therefore, it’s worth to investigate the immune-regulatory effects of Djulis. Moreover, the Amaranthaceae family, including Djulis, quinoa and amaranthus seed, has similarity appearance, thereby is hard to discriminate between them. To characterize the chemical profile of Djulis as the standardization is very important. The aim of this project is to investigate the immune-regulatory effects of Djulis and its bioactive components using metablolmics. First, to evaluate the immune-regulatory effects of Djulis in cell model, and to isolation and purification the bioactive compounds from Djulis. Second, the chemical profile of Djulis was characterized by the fingerprint of HPLC/MS. Furthermore, the metabolomic study was launched by chemometric analysis to distinguish the different among Djulis, quinoa and amaranthus seed. Third, the effects of the standardized Djulis were carried out in accordance with the assessment method of health food on immune function in vivo. In addition, the 28-day feeding toxicity study was performed to estimate the no-observed-adverse-effect level of Djulis. These results will provide as evidenced-based reference to promote the application of Djulis in the future.臺灣紅藜藜麥甜菜色素免疫調節代謝體學Djulisquinoabetalainsimmune-regulatorymetabolomics以代謝體學探討臺灣紅藜調節免疫之功效評估及其活性成分