國立臺灣大學植物病理與微生物學系暨研究所林長平2006-07-262018-06-292006-07-262018-06-292002http://ntur.lib.ntu.edu.tw//handle/246246/17684The PCR-based strategy was used for cloning and analysis of dnaK gene of phytoplasma associated with peanut witchs’ broom. For nested-PCR, two pairs of nucleotide primer were designed based on the conserved regions of bacterial dnaK genes. A 750 bp dnaK gene fragment was amplified as predicted and further used for screening an EcoRI-digested genomic library of PNWB phytoplasma. Four open reading frames were identified in the order of ORF1, grpE, dnaK and dnaJ . Chromosomal arrangement of these genes in phytoplasmas is identical to Clostridium acetobutylium and other bacteria phylogenic closed to phytoplasmas. It implies the primitive bacterial molecular chapone HSP70 mechine exsists in phytoplasma and the mechanism it may involve when phytoplasma exposed to stress could be similar to other prokaryotes.application/pdf193734 bytesapplication/pdfzh-TW國立臺灣大學植物病理與微生物學系暨研究所Heat shock proteinMollicutesnested-PCRotherhttp://ntur.lib.ntu.edu.tw/bitstream/246246/17684/1/902313B002321.pdf