Huang, JPJPHuangHan, YSYSHanTzeng, WNWNTzeng2008-04-102018-07-062008-04-102018-07-062001http://ntur.lib.ntu.edu.tw//handle/246246/64328Gonadotropin II beta-subunit gene (GTH II-β DNA) fragments of nine eel species of the genus Anguilla were amplified with specific oligonucleotides by polymerase chain reaction (PCR), and a single product was obtained from each species. In subsequent restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified GTH II-β DNA, the restriction endonuclease Hpa II, Sph I and Nsi I gave restriction patterns that differed among 3 species, e.g., Anguilla japonica, A. bicolor bicolor and A. anguilla, with other six species of anguillid eels, e.g., A. rostrata, A. australis australis, A. reinhardti, A. celebesensis, A. marmorata, and A. bicolor pacifica. Our results suggest that the PCR/RFLP analysis provides a rapid and convenient method to discriminate eel species.361772 bytesapplication/pdfen-USgenus Anguilla, gonadotropin II beta-subunit gene (GTH II-β), PCR, RFLP, restriction enzymehttp://ntur.lib.ntu.edu.tw/bitstream/246246/64328/1/eel-PCR-RFLP-paper AZT.pdf