2019-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/674643摘要：馬鈴薯為世界上重要作物之一。其於台灣種植面積約2千2百公頃，年產出約5萬2千公噸，產值約六億新台幣。台灣馬鈴薯種植區域主要分佈於台中市、雲林縣及嘉義縣。馬鈴薯瘡痂病主由馬鈴薯瘡痂病菌Streptomyces scabies造成，嚴重影響馬鈴薯經濟價值。然而，目前並無官方推薦之防治方法。我們實驗室今年發表一株液化澱粉芽孢桿菌(Bacillus amyloliquefaciens)Ba01能於盆栽及田間試驗有效抑制馬鈴薯瘡痂病，其可能抑病機制是透過脂&#32957;類生物界面活性劑,伊枯草菌素A及豐原素之分泌。然而，更詳細之抑病機制仍不清楚。我們因此擬提三研究目標來探討抑病機制。研究目標一：發現液化澱粉芽孢桿菌抑制馬鈴薯瘡痂病菌之生長與發育之基因。研究目標二：探討液化澱粉芽孢桿菌抑制馬鈴薯瘡痂病菌之機制。研究目標三：分析液化澱粉芽孢桿菌突變株防治馬鈴薯瘡痂病之功效。此研究計畫不僅能找出Ba01調控馬鈴薯瘡痂病菌生長與發育相關基因，亦能解析其防治馬鈴薯瘡痂病之分子機制。<br> Abstract: Potato is one of the most important crops worldwide, while it annually produces ~52,000 tons (costing 600 million NT$) in ~2,200 hectares in Taiwan. These potato fields mainly distributed in Taichung, Yunlin and Chiayi of Taiwan. Potato common scab caused by Streptomyces scabies is a severe disease and causes enormous economic lost and currently there is no officially recommended control agents. In 2018, our lab published that biocontrol agent Bacillus amyloliquefaciens (Ba) Ba01 can control potato common scab in pot assays and a nature occurring field, and the mechanism is potentially via the secretion of surfactin, iturin A and fengycin. However, the mechanisms that Ba controls potato common scab remained unclear in molecular level. We thus propose three specific aims to identify mechanisms. Specific aim 1: Discovery of Ba genes involved in controlling the growth and development of S. scabies. Specific aim 2: Investigate the mechanisms of Ba used to control S. scabies. Specific aim 3: Analyze the control efficacy of Ba mutants against potato common scab. Our proposal not only identify Ba genes involved in controlling the growth and development of S. scabies, but also elucidates the mechanisms in molecular level that Ba01 controls potato common scab.馬鈴薯馬鈴薯瘡痂病馬鈴薯瘡痂病菌液化澱粉芽孢桿菌抑病機制potatopotato common scabStreptomyces scabiesBacillus amyloliquefaciensinhibition mechanism