泌尿科PU, YEONG-SHIAUYEONG-SHIAUPULUO, WEIPINGWEIPINGLUOLU, HSIO-HUEIHSIO-HUEILUGREENBERG, NORMAN M.NORMAN M.GREENBERGLIN, SUE-HWASUE-HWALIN2009-01-162018-07-112009-01-162018-07-111999http://ntur.lib.ntu.edu.tw//handle/246246/97102Purpose: The transgenic adenocarcinoma of mouse prostate ( TRAMP) model, in which various grades of prostate intraepithelial neoplasia (PIN) and prostate cancer with metastases can be reproducibly generated, is a paradigm for prostate disease progression. We have previously shown that C -CAM, an adhesion molecule, can suppress the growth of prostate cancer. In this report, we describe immunohistochemical characterization of differential expression of C-CAM at various stages of prostate tumorigenesis in the TRAMP model. Materials and Methods: We sampled prostate specimens and periaortic lymph nodes from TRAMP mice. Indirect immunohistochemical staining with a polyclonal anti-C-CAM antibody was performed on the formalin -fixed, paraffin-embedded specimens. After castration at 12 weeks of age, the TRAMP mice developed androgen- independent prostate cancer (AIPC) and lymph node metastasis at 18 to 24 weeks of age. Samples from these castrated mice were also analyzed. Results: C-CAM protein was expressed in the normal prostate epithelia of non-transgenic and TRAMP mice as well as in low-grade PINs in TRAMP mice. Expression was uniform on the luminal surfaces of these epithelia. C-CAM expression was noticeably reduced and the staining pattern heterogeneous in some high-grade PINs. C-CAM staining was generally absent in prostate cancer and metastatic lymph nodes. Androgen independent prostate cancer and its metastatic tumors generated in castrated TRAMP mice were also C- CAM negative. Conclusions: C-CAM expression correlates with the differentiation states of prostate epithelia and is down regulated early in prostate tumorigenesis in the TRAMP model.en-UStransgenic miceprostatic neoplasmsANTIGEN GENE FAMILYCOLORECTAL CARCINOMASBILIARY GLYCOPROTEIN[SDGs]SDG3