Inserra C.Labelle P.Der Loughian C.Lee J.-L.Fouqueray M.Ngo J.Poizat A.Desjouy C.Munteanu B.Lo C.-W.Vanbelle C.Rieu J.-P.WEN-SHIANG CHENBéra J.-C.2020-03-302020-03-3020141959-0318https://www.scopus.com/inward/record.uri?eid=2-s2.0-84898413632&doi=10.1016%2fj.irbm.2014.02.010&partnerID=40&md5=de22768296a3b3c549bdbce8eafc89c7https://scholars.lib.ntu.edu.tw/handle/123456789/481421Sonoporation process, at the origin of ultrasound cell transfection, is ruled by the interaction between cells and cavitating bubbles. Due to the stochastic behavior of acoustic cavitation, there exists a need in ensuring a stable state of cavitation within a medium during cell transfection to enhance transfection efficiency and control mortality. The goal of the SonInCaRe project is thus to define a controlled-cavitation device in order to monitor, control and stabilize inertial cavitation activity during cell sonication in real-time. This device, based on a feedback loop acting in real-time, allows ensuring fixed cavitation conditions during a pulsed sonication. Its application to suspended and adherent cells transfection shows better reproducibility compared to the fixed acoustic intensity sonication. The regulation device thus provides a better control of cavitation activity and its bioeffects which are of crucial importance for clinical applications of ultrasound-mediated gene transfection. ? 2014 Elsevier Masson SAS.[SDGs]SDG3adherent cell; article; feedback system; genetic transfection; human; human cell; lipid bilayer; oscillation; reproducibility; sound intensity; ultrasound; ultrasound cell transfectionjournal article10.1016/j.irbm.2014.02.0102-s2.0-84898413632