2008-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/692583摘要：隨著高解析度定位選殖數量性狀基因座的研究增加，傳統的數量性狀基因座以複合式基因組形式存在的範例有逐漸增加的趨勢。深入研究此數量性狀基因座的新特性或許有助於啟發未來作物育種的新思維。蕃茄柱頭外凸的主效數量性狀基因座即為一複合式基因組，其中包括了至少五個連鎖的基因，一個控制雌蕊的長度，三個控制雄蕊的長度，另一個則影響雄蕊尖端的彎曲。為了了解複合式基因組中基因之間的關係，我們擬於未來的三年中，先進行高解析度定位選殖兩個緊密連鎖且都控制雄蕊長度的基因stamen 2.2 與 stamen 2.3。本研究計畫將採用區間取代定位法進行高解析度定位選殖。計畫內容包括兩類主要工作: 一是要得到位於介於分子標誌 cLED19A24 與 CT9 之間完整的基因體核酸序列，以便用來發展新的分子標誌；另一工作是產生進行區間取代定位法的遺傳定位族群。大規模重組植株的篩選、決定重組系的染色體交換位址、及進行大規模田間試驗以紀錄外表性狀，此三者是達成定位選殖成功的要件。<br> Abstract: With the advent of high-resolution mapping of quantitative trait locus, increasing cases showed that a quantitative trait locus is actually a compound locus. Studying this new characterization of quantitative trait locus might shed light on the new era of plant breeding. The stigma exsertion 2.1 QTL is a compound locus, comprising at least five tightly linked genes, one controlling style length, three controlling stamen length, and the other affecting the curvature of the stamen tips. In order to understand the relationships among these genes, we aim to positional clone the two closely linked stamen gene, stamen2.2 and stamen2.3. Substitution mapping will be used as the strategy to positional clone the stamen2.2 and stamen2.3 genes. Two major works are included: First, to identify the whole genomic sequences between the molecular markers cLED24A19 and CT9; Second, to create the mapping population for substitution mapping. There are three major goals for the success of positional cloning: a large scale screening of recombinants, to determine the crossover site of all screened recombinants, and the final evaluation of phenotypes in the field.複合式基因組數量性狀基因座定位選殖番茄雄蕊長度。compound locusquantitative trait locuspositional cloningtomatostamen length高解析度定位位於se2.1數量基因座中控制蕃茄雄蕊長度的基因群