JULIA YU-FONG CHANGLin M.-C.CHUN-PIN CHIANG2021-10-152021-10-1520030002-9173https://www.scopus.com/inward/record.uri?eid=2-s2.0-0345258410&doi=10.1309%2fC5P6NUQ2NW6LCTBP&partnerID=40&md5=013d3c57f1e3301e0b76ef8902e8e7f2https://scholars.lib.ntu.edu.tw/handle/123456789/584897To evaluate the etiologic role of human papillomavirus (HPV) in oral carcinogenesis, DNA samples were purified from 103 oral squamous cell carcinoma (OSCC) and 30 normal oral mucosal (NOM) specimens. A nested polymerase chain reaction, DNA sequencing, and gene-chip HPV typing were used to identify multiple HPV types in our samples. We found that the positive rates of all HPV types and of high-risk HPV types were significantly higher in OSCC samples (49.5% and 41.7%, respectively) than in NOM samples (6/30 [20%; P < .01] and 5/30 [17%; P < .05], respectively) and significantly higher in non-oral habits (OH)-associated OSCC samples (31/51 [61%] and 28/51 [55%], respectively) than in OH-associated OSCC samples (20/52 [38%; P < .05] and 15/52 [29%; P < .001], respectively). High-risk HPV types and all HPV types had odds ratios of 3.97 (P = .0097) and 3.92 (P = .006), respectively. Our results suggest that HPVs, particularly high-risk HPVs, might be associated with the development of OSCCs, especially the non-OH-associated OSCCs.High-risk HPV types; HPV; Human papillomavirus; Oral cancer[SDGs]SDG3DNA; adult; aged; article; carcinogenesis; controlled study; DNA sequence; female; human; human tissue; major clinical study; male; mouth carcinoma; mouth mucosa; nonhuman; Papilloma virus; polymerase chain reaction; priority journal; virus identification; virus typingjournal article10.1309/C5P6NUQ2NW6LCTBP146719802-s2.0-0345258410