SHANG-TZEN CHANGShyur, Lie-FenLie-FenShyurHuang, Chi-ChangChi-ChangHuangLo, Chiu-PingChiu-PingLoChiu, Chih-YangChih-YangChiuChen, Yi-PingYi-PingChenWang, Sheng-YangSheng-YangWang2018-09-102018-09-102008http://www.scopus.com/inward/record.url?eid=2-s2.0-41049090217&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/338514Cryptomeria japonica is an important plantation conifer tree in Asia. This study aimed to characterize the anti-inflammatory and hepatoprotective activities of the phytocompounds from C. japonica wood on LPS- or TPA-induced activation of proinflammatory mediators and CCl4-induced acute liver injury in mice. A CJH7-2 fraction was purified from C. japonica extracts following bioactivity-guided fractionation, and it exhibited significant activities on inhibition of NO production and iNOS expression as well as up-regulating HO-1 expression in LPS-stimulated macrophages. CJH7-2 also potently inhibits COX-2 enzymatic activity (IC50 = 5 μg/mL) and TPA-induced COX-2 protein expression in mouse skin (1 mg/200 μL/site). CJH7-2 (10 mg/kg BW) can prevent CCl4-induced liver injury and aminotransferases activities in mice. Chemical fingerprinting analysis showed that terpenes are the major bioactive compounds in the CJH7-2 fraction. This is the first study to demonstrate that chemical constituents from the wood extract of C. japonica possess anti-inflammatory activities in vitro and in vivo that may play a role in hepatoprotection. ? 2008 Elsevier Ltd. All rights reserved.965892 bytesapplication/pdfCryptomeria japonica; Cycloxygenase-2; Heme oxygenase-1; Hepatoprotection; Inducible nitric oxide synthase; Nitric oxide; Taxodiaceae; Terpenoids[SDGs]SDG3carbon tetrachloride; prostaglandin synthase; prostaglandin synthase inhibitor; terpene; animal; article; cell line; chemical structure; chemically induced disorder; chemistry; drug effect; female; inflammation; Institute for Cancer Research mouse; Japanese cedar; liver disease; macrophage; male; metabolism; mouse; skin; wood; Animals; Carbon Tetrachloride; Cell Line; Cryptomeria; Cyclooxygenase Inhibitors; Female; Inflammation; Liver Diseases; Macrophages; Male; Mice; Mice, Inbred ICR; Molecular Structure; Prostaglandin-Endoperoxide Synthases; Skin; Terpenes; Wood; Coniferophyta; Cryptomeria japonica; Cupressaceae; Musjournal article10.1016/j.phytochem.2008.01.017